Bloodstains found at a crime scene can help estimate the events that occurred during the crime. Reconstructing the crime scene by analyzing the bloodstain pattern contributes to understanding the bloody event. Therefore, it is essential to classify bloodstains through bloodstain pattern analysis (BPA) and accurately estimate the actions that took place at that time.
View Article and Find Full Text PDFBackground: Renal replacement therapy (RRT) may affect coagulation and platelet function in critically ill patients. However, the mechanism and the difference in the impact on coagulation between intermittent hemodialysis (iHD) and continuous renal replacement therapy (CRRT) remains unclear. This study aimed to investigate and compare the impact of iHD and CRRT on coagulation and platelet function.
View Article and Find Full Text PDFIn digital pathology, whole slide images (WSI) are crucial for cancer prognostication and treatment planning. WSI classification is generally addressed using multiple instance learning (MIL), alleviating the challenge of processing billions of pixels and curating rich annotations. Though recent MIL approaches leverage variants of the attention mechanism to learn better representations, they scarcely study the properties of the data distribution itself i.
View Article and Find Full Text PDFBiochem Biophys Res Commun
November 2024
Abnormal adipose tissue formation is associated with metabolic disorders such as obesity, diabetes, and liver and cardiovascular diseases. Thus, identifying the novel factors that control adipogenesis is crucial for understanding these conditions and developing targeted treatments. In this study, we identified the melanosome-related factor MLPH as a novel adipogenic factor.
View Article and Find Full Text PDFCell surface display engineering facilitated the development of a cobalt-binding hybrid . OmpC served as the molecular anchor for showcasing the cobalt-binding peptides (CBPs), creating the structural model of the hybrid OmpC-CBPs (OmpC-CP, OmpC-CF). Subsequently, the recombinant peptide's cobalt adsorption and retrieval effectiveness were evaluated at various concentrations.
View Article and Find Full Text PDFEscherichia coli were engineered to selectively adsorb and recover lithium from the environment by employing a bacterial cell surface display strategy. Lithium binding peptide (LBP1) was integrated into the Escherichia coli membrane protein OmpC. The effect of environmental conditions on the adsorption of lithium by a recombinant strain was evaluated, and lithium particles on the cellular surface were analyzed by FE-SEM and XRD.
View Article and Find Full Text PDFThe aim of this study was to investigate the anti-angiogenic effects of the hexane fraction of var. root extract (HAT) and its influence on the development of erlotinib resistance in human lung cancer cells. HAT significantly reduced the migration, invasion, and tube formation of human umbilical vein endothelial cells (HUVECs).
View Article and Find Full Text PDFThe condition of a railway vehicle's wheels is an essential factor for safe operation. However, the current inspection of railway vehicle wheels is limited to periodic major and minor maintenance, where physical anomalies such as vibrations and noise are visually checked by maintenance personnel and addressed after detection. As a result, there is a need for predictive technology concerning wheel conditions to prevent railway vehicle damage and potential accidents due to wheel defects.
View Article and Find Full Text PDFThe wheels of railway vehicles are of paramount importance in relation to railroad operations and safety. Currently, the management of railway vehicle wheels is restricted to post-event inspections of the wheels whenever physical phenomena, such as abnormal vibrations and noise, occur during the operation of railway vehicles. To address this issue, this paper proposes a method for predicting abnormalities in railway wheels in advance and enhancing the learning and prediction performance of machine learning algorithms.
View Article and Find Full Text PDFNeuroligins (NLGNs) are postsynaptic cell adhesion molecules that are involved in synapse assembly and function. The NLGN gene family consists of 5 genes (, , and ). NLGN3 forms heterodimers with other NLGNs and is expressed at both excitatory and inhibitory synapses, although the distinct role at different synapses is not fully understood.
View Article and Find Full Text PDFIn this study, we developed a substrate-independent initiator film that can undergo surface-initiated polymerization to form an antifouling brush. Inspired by the melanogenesis found in nature, we synthesized a tyrosine-conjugated bromide initiator (Tyr-Br) that contains phenolic amine groups as the dormant coating precursor and α-bromoisobutyryl groups as the initiator. The resultant Tyr-Br was stable under ambient air conditions and underwent melanin-like oxidation only in the presence of tyrosinase to form an initiator film on various substrates.
View Article and Find Full Text PDFPrevious studies have indicated that the adrenergic receptor signaling pathway plays a fundamental role in chronic stress-induced cancer metastasis. In this study, we investigated whether an ethanol extract of leaves (EPF) traditionally used to treat stress-related symptoms by moving Qi could regulate the adrenergic agonist-induced metastatic ability of cancer cells. Our results show that adrenergic agonists including norepinephrine (NE), epinephrine (E), and isoproterenol (ISO) increased migration and invasion of MDA-MB-231 human breast cancer cells and Hep3B human hepatocellular carcinoma cells.
View Article and Find Full Text PDFPhytochrome B (phyB) is a plant photoreceptor that forms a membraneless organelle called a photobody. However, its constituents are not fully known. Here, we isolated phyB photobodies from Arabidopsis leaves using fluorescence-activated particle sorting and analyzed their components.
View Article and Find Full Text PDFEye-gaze direction-tracking technology is used in fields such as medicine, education, engineering, and gaming. Stability, accuracy, and precision of eye-gaze direction-tracking are demanded with simultaneous upgrades in response speed. In this study, a method is proposed to improve the speed with decreases in the system load and precision in the human pupil orbit model (HPOM) estimation method.
View Article and Find Full Text PDFRecently, autonomous driving technology has been in the spotlight. However, autonomous driving is still in its infancy in the railway industry. In the case of railways, there are fewer control elements than autonomous driving of cars due to the characteristics of running on railways, but there is a disadvantage in that evasive maneuvers cannot be made in the event of a dangerous situation.
View Article and Find Full Text PDFChronic stress has been reported to stimulate the release of catecholamines, including norepinephrine (NE) and epinephrine (E), which promote cancer progression by activating the adrenergic receptor (AR). Although previous studies showed that β2-AR mediated chronic stress-induced tumor growth and metastasis, the underlying mechanism has not been fully explored. In this study, we aimed to investigate the molecular mechanism by which β2-AR exerts a pro-metastatic function in hepatocarcinoma (HCC) cells and breast cancer (BC) cells.
View Article and Find Full Text PDFInspired by the melanogenesis occurring in nature, we report tyrosinase-mediated antifouling surface coating by synthesizing a tyrosine-conjugated sulfobetaine derivative (Tyr-SB). Synthetic Tyr-SB contains zwitterionic sulfobetaine and tyrosine, whose phenolic amine group acts as a dormant coating precursor. In contrast to catecholamine derivatives, tyrosine derivatives are stable against auto-oxidation and are enzymatically oxidized only in the presence of tyrosinase to initiate melanin-like oxidation.
View Article and Find Full Text PDFThe survival and death of eukaryotic cells are tightly controlled by a variety of proteins in response to the cellular environment. Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) is a receptor-interacting Ser/Thr kinase that has recently been reported as an important regulator of cell survival, apoptosis, and necroptosis; however, its role in liver cancer remains unclear. In this study, we examined the effect of siRNA-mediated RIPK1 knockdown on the survival and death of liver cancer cells.
View Article and Find Full Text PDFAnemia is associated with physical, cognitive, and developmental problems. Given that there are limited studies on anemia prevalence among obese Asian adolescents and that past evidence is controversial, this study investigated the relationship between obesity and anemia in a nationally representative sample of South Korean adolescents. Data were obtained from the 2007-2019 Korea National Health and Nutrition Examination Survey.
View Article and Find Full Text PDFIn digital pathology, segmentation is a fundamental task for the diagnosis and treatment of diseases. Existing fully supervised methods often require accurate pixel-level annotations that are both time-consuming and laborious to generate. Typical approaches first pre-process histology images into patches to meet memory constraints and later perform stitching for segmentation; at times leading to lower performance given the lack of global context.
View Article and Find Full Text PDFThe purpose of this paper is to study the recognition of ships and their structures to improve the safety of drone operations engaged in shore-to-ship drone delivery service. This study has developed a system that can distinguish between ships and their structures by using a convolutional neural network (CNN). First, the dataset of the Marine Traffic Management Net is described and CNN's object sensing based on the Detectron2 platform is discussed.
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