Publications by authors named "Jaeho Paek"
G-protein-coupled receptors (GPCRs) play critical roles in regulating physiological processes ranging from neurotransmission to cardiovascular function. Current methods for tracking GPCR signaling suffer from low throughput, modification or overexpression of effector proteins, and low temporal resolution. Here, we show that peroxidase-catalyzed proximity labeling can be combined with isobaric tagging and mass spectrometry to enable quantitative, time-resolved measurement of GPCR agonist response in living cells.
View Article and Find Full Text PDFCopper is an endogenous modulator of neural circuit spontaneous activity.
Proc Natl Acad Sci U S A
November 2014
Article Synopsis
- The brain requires high levels of metals for proper functioning, and while alkali and alkaline earth metals are needed for signaling, transition metals like copper are often thought to be stored in static reservoirs.
- Researchers developed a new copper sensor (Copper Fluor-3) that reveals neurons maintain accessible, loosely bound copper stores, which can be affected by chelation.
- Disrupting these copper reserves through chelation or genetic modifications negatively impacts the spontaneous activity in neural circuits, highlighting copper's crucial role in neuronal function and signaling.