gen. nov., sp. nov., a Novel Genus within the Family , Was Isolated from Brackish Water in the Republic of Korea.

A Gram-stain-negative, obligately aerobic, non-motile, rod-shaped bacterial strain designated SJW1-29 was isolated from brackish water samples collected from the Seomjin River, Republic of Korea. The purpose of this study was to characterize strain SJW1-29 and determine its taxonomic position as a potential new genus within the family . The strain grew within the range of 10-30 °C (optimum, 25 °C), pH 5.

-Mediated Transformation of the Aquatic Fungus FBCC-F1546.

is a useful fungus known for its ability to mineralise lignin during primary metabolism and decompose polycyclic aromatic hydrocarbons (PAHs). However, no functional genetic analysis techniques have been developed yet for this fungus, specifically in terms of transformation. In this study, we applied an -mediated transformation (ATMT) system to for a functional gene analysis.

Genome sequence of freshwater sediment isolated fungus strain NNIBRFG27121.

We sequenced the genome of NNIBRFG27121 strain isolated from the wetland of Maehwamarum Habitat in Korea. The final assembly consisted of six scaffolds with a size of 31.96 Mb and an N50 of 8,770,400 bp.

Comparative Pathogenicity and Host Ranges of and Related Species.

Host shifting and host expansion of fungal plant pathogens increases the rate of emergence of new pathogens and the incidence of disease in various crops, which threaten global food security. species cause serious disease in rice, namely rice blast disease, as well as in many alternative hosts, including wheat, barley, and millet. A severe outbreak of wheat blast due to occurred recently in Bangladesh, after the fungus was introduced from South America, causing great loss of yield.

Isolation and Characterization of Four Unrecorded Species in Korea.

During an investigation of fungi of the order Mucorales from freshwater and sediment samples in Korea, we isolated six strains, NNIBRFG6649, NNIBRFG6255, NNIBRFG1498, CNUFC-YJ13, CNUFC-YR7, and NNIBRFG2739. The morphology and phylogeny of these strains were analyzed. Based on the morphological characteristics and molecular data from internal transcribed spacer (ITS) region, the isolates NNIBRFG6649 and NNIBRFG6255 were identified as , and .

Draft Genome Sequence of the Aquatic Fungus Strain NNIBRFG339.

is an aquatic fungal species found in leaf litter. Here, we report the 42.5-Mb draft genome sequence of strain NNIBRFG339, which comprises 61 scaffolds and has an overall G+C content of 45.

Draft Genome Sequence of the Decomposition Fungus Strain NNIBRFG19.

Aquanectria penicillioides is a common aquatic fungal species. Here, we report the 53.7-Mb draft genome sequence of strain NNIBRFG19, which has an overall G+C content of 47.

Draft Genome Sequence of the Aquatic Fungus Strain NNIBRFG329.

Hymenoscyphus tetracladius (anamorph, Articulospora tetracladia) is a common aquatic hyphomycetous fungus. Here, we report the draft genome sequence of H. tetracladius strain NNIBRFG329, which comprises 41.

ER retention receptor, MoERR1 is required for fungal development and pathogenicity in the rice blast fungus, Magnaporthe oryzae.

ER retention receptor is a seven trans-membrane protein that plays pivotal roles in function and integrity of endoplasmic reticulum (ER). Insertional mutagenesis of Magnaporthe oryzae identified MoERR1 as a pathogenicity gene encoding putative ER retention receptor orthologous to ERD2 in Saccharomyces cerevisiae. Search through the genome identified that M.

The PEX7-mediated peroxisomal import system is required for fungal development and pathogenicity in Magnaporthe oryzae.

In eukaryotes, microbodies called peroxisomes play important roles in cellular activities during the life cycle. Previous studies indicate that peroxisomal functions are important for plant infection in many phytopathogenic fungi, but detailed relationships between fungal pathogenicity and peroxisomal function still remain unclear. Here we report the importance of peroxisomal protein import through PTS2 (Peroxisomal Targeting Signal 2) in fungal development and pathogenicity of Magnaporthe oryzae.

The cell cycle gene MoCDC15 regulates hyphal growth, asexual development and plant infection in the rice blast pathogen Magnaporthe oryzae.

Rice blast, caused by the pathogen Magnaporthe oryzae, is a serious hindrance to rice production and has emerged as an important model for the characterization of molecular mechanisms relevant to pathogenic development in plants. Similar to other pathogenic fungi, conidiation plays a central role in initiation of M.oryzae infection and spread over a large area.

Homeobox transcription factors are required for conidiation and appressorium development in the rice blast fungus Magnaporthe oryzae.

The appropriate development of conidia and appressoria is critical in the disease cycle of many fungal pathogens, including Magnaporthe oryzae. A total of eight genes (MoHOX1 to MoHOX8) encoding putative homeobox transcription factors (TFs) were identified from the M. oryzae genome.

A putative MAP kinase kinase kinase, MCK1, is required for cell wall integrity and pathogenicity of the rice blast fungus, Magnaporthe oryzae.

Insertional mutagenesis of Magnaporthe oryzae led to the identification of MCK1, a pathogenicity gene predicted to encode mitogen-activated protein kinase kinase kinase (MAPKKK) homologous to BCK1 in Saccharomyces cerevisiae. Targeted disruption of MCK1 resulted in the fungus undergoing autolysis and showing hypersensitivity to cell-wall-degrading enzyme. The mck1 produced significantly reduced numbers of conidia and developed appressoria in a slightly retarded manner compared with the wild type.

Genome-wide analysis of T-DNA integration into the chromosomes of Magnaporthe oryzae.

Agrobacterium tumefaciens-mediated transformation (ATMT) has become a prevalent tool for functional genomics of fungi, but our understanding of T-DNA integration into the fungal genome remains limited relative to that in plants. Using a model plant-pathogenic fungus, Magnaporthe oryzae, here we report the most comprehensive analysis of T-DNA integration events in fungi and the development of an informatics infrastructure, termed a T-DNA analysis platform (TAP). We identified a total of 1110 T-DNA-tagged locations (TTLs) and processed the resulting data via TAP.

Genome-wide functional analysis of pathogenicity genes in the rice blast fungus.

Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae.