Segmented Two-Dimensional Progressive Polynomial Calibration Method for Nonlinear Sensors.

Nonlinearity in sensor measurements reduces the sensor's accuracy. Therefore, accurate calibration is necessary for reliable sensor operation. This study proposes a segmented calibration method that divides the input range into multiple sections and calculates the optimized calibration functions for each one.

Corneal lymphangiogenesis facilitates ocular surface inflammation and cell trafficking in dry eye disease.

Purpose: While the normal cornea has limited innervation by the lymphatic system, chronic immune-inflammatory disorders such as dry eye (DE) can induce lymphangiogenesis in the ocular surface. Using a conditional knock-down murine model, Lyve-1;VEGFR2 mice, this study investigated the role of lymphangiogenesis in the pathophysiology of DE.

Methods: DE was induced in both wild type (WT) B6 and Lyve-1;VEGFR2 mice.

Efficient cultivation conditions for human limbal epithelial cells.

To compare the stem niche in different culture conditions of limbal epithelial cells, the suspended human limbal epithelial cells (HLECs) were seeded on the 3T3-pretreated plates and the other suspended cells were plated on amniotic membranes (AMs) which were either cryo-preserved or freeze-dried. All were cultured for 10 to 12 days. Reverse transcription-polymerase chain reaction (RT-PCR) for ATP-binding cassette, subfamily G, member 2 (ABCG2), p63, cytokeratin 12, and connexin 43 were performed in cultivated HLECs and their expression levels were compared.

Short-term Efficacy of Topical Immunosuppressive Agents on the Survival of Cultivated Allo-Conjunctival Equivalents.

Purpose: To investigate the short-term efficacy of topical immunosuppressive agents on the survival of cultivated allo-conjunctival equivalents.

Methods: Twenty-five eyes of New Zealand white rabbits were included. Temporal conjunctivae were trephined to a diameter of 7.

Experience of Comamonas acidovorans keratitis with delayed onset and treatment response in immunocompromised cornea.

Purpose: To report 2 cases of Comamonas acidovorans keratitis in immunocompromised cornea.

Methods: A complete review of the medical records of the two cases of Comamonas acidovorans keratitis.

Results: We found some similarities in clinical courses of two cases.

Clinical efficacy of amniotic membrane transplantation in the treatment of various ocular surface diseases.

Purpose: To investigate the efficacy of permanent amniotic membrane transplantation (AMT) for the treatment of various ocular surface diseases.

Methods: The medical records of 62 eyes from 58 patients who had undergone permanent AMT were reviewed. The amniotic patches were grafted for the treatment of neurotrophic ulcers (n=15), inflammatory corneal ulcers (n=15), scleral ulcers (n=11), painful bullous keratopathy (n=8) and pterygium as an adjuvant to a conjunctival autograft (n=13).

Efficient cryopreservative conditions for cultivated limbal and conjunctival epithelial cells.

Purpose: To examine the effects of cryopreservation on the viability of cultivated corneal limbal and conjunctival epithelial cells and to evaluate the optimal conditions for cryopreservation.

Methods: The cultivated human limbal epithelial cells (HLECs) were stored in media including 20%, 50%, and 90% fetal bovine serum (FBS) and 10% dimethyl sulfoxide (DMSO) at -196 degrees C for 1 week. The cultivated rabbit conjunctival epithelial cells were stored in 10%, 20%, and 50% FBS with 10% glycerol or DMSO as a cryoprotectant at -196 degrees C for 1 week.

Long-term outcome in ocular intractable surface disease with Seoul-type keratoprosthesis.

Purpose: To evaluate the long-term clinical efficacy of the Seoul-type keratoprosthesis (S-KPro).

Methods: S-KPros were implanted into 4 unsighted and 5 sighted eyes in 9 patients: 6 patients were diagnosed with Stevens-Johnson syndrome, 2 had chemical burns, and 1 suffered from ocular pemphigoid. The preoperative visual acuity ranged from light perception to hand motion.

Biocompatibility of nanocomposites used for artificial conjunctiva: in vivo experiments.

Purpose: To evaluate the biocompatibility of nanocomposites used for artificial conjunctiva.

Methods: Fifty New Zealand white rabbits were used for the experiments. Nanocomposites of poly -caprolactone (PCL) and of PCL coated with polyvinyl alcohol (PCL+PVA), polyvinyl pyrrolidone (PCL+PVP), or chitosan (PCL+C), and amniotic membrane (AM) as a control, were cut into small disks with a diameter of 3.

Isolation of putative corneal epithelial stem cells from cultured limbal tissue.

Purpose: To investigate methods of isolating putative corneal epithelial stem cells from cultured limbal tissue.

Methods: Three extraction techniques were compared to identify an efficient method of obtaining a large number of viable corneal epithelial stem cells from the limbus. Limbal tissues were extracted by incubation at 37 degrees C or 4 degrees C for 1 or 16 hours, respectively, with 1.

The side population cells in the rabbit limbus sensitively increased in response to the central cornea wounding.

Purpose: Side population (SP) cells are known to reside in the limbus as putative corneal epithelial stem cells. This study was performed to demonstrate the presence and the characteristics of SP cells in the rabbit limbal epithelium and explore their sensitivity in response to the central cornea wounding.

Methods: To sort out the SP cells, freshly isolated rabbit limbal and central corneal epithelial cells were subjected to Hoechst 33342 dye efflux assay.

Adhesion complex in cultivated limbal epithelium on amniotic membrane after in vivo transplantation.

Purpose: To investigate adhesion complex formation in cultivated human limbal epithelium after transplantation into the limbal deficient model.

Methods: Cultivated epithelium on amniotic membrane was transplanted into limbal deficient rabbits. The transplanted rabbits and the controls were sacrificed at 1, 2, 3, and 4 weeks.

Change of proliferation rate of corneal epithelium in the rabbit with orthokeratology lens.

Objective: To investigate the cell proliferation rate of normal corneal epithelium with extended orthokeratology lens (OKL) wear in comparison with extended rigid gas-permeable (RGP) lens wear.

Methods: Twenty-four rabbits were fitted unilaterally with either an OKL or an RGP lens, and the other eye was used as a control. They were injected with 5-bromo-2-deoxyuridine (BrdU) 24 h prior to being sacrificed.

Optimization of contact lens fitting in keratectasia patients after laser in situ keratomileusis.

Purpose: To evaluate the clinical outcomes of contact lens fitting in eyes with keratectasia after laser in situ keratomileusis (LASIK) to optimize the performance of the contact lenses.

Setting: Contact Lens Clinic, Seoul National University Hospital, Seoul, Korea.

Methods: Six eyes of 4 patients with keratectasia were examined.

Clinical performance and fitting characteristics with a multicurve lens for keratoconus.

Purpose: To evaluate the clinical outcome and fitting characteristics with a multicurve lens for keratoconus in a Korean population.

Methods: Sixty eyes with keratoconus were fit with a multicurve lens based on topographic index and fluorescein pattern on slitlamp biomicroscopy. Three-point touch was applied in 59 eyes, and apical clearance was adopted in an eye with a persistent epithelial defect.

Seoul-type keratoprosthesis: preliminary results of the first 7 human cases.

Objective: To evaluate the clinical efficacy of a newly designed Seoul-type keratoprosthesis (S-KPro).

Methods: The S-KPro, which consists of a polymethyl methacrylate optic, a skirt (polyurethane or polypropylene), and polypropylene haptics, was developed and implanted into 2 unsighted and 5 sighted eyes of 7 patients. One patient had a chemical burn, another had an ocular pemphigoid, and the remainder were diagnosed as having Stevens-Johnson syndrome.