Publications by authors named "Jae-Bang Choi"
Article Synopsis
- The study aimed to enhance the production of virus-like particles (VLPs) for enterovirus 71 (EV71), which causes hand, foot, and mouth disease (HFMD).
- Researchers focused on optimizing the expression levels of the P1 precursor protein and the 3CD protein necessary for cleaving P1, adjusting factors like promoter strength.
- Findings showed that using a specific weakened promoter led to effective P1 and 3CD expression, resulting in high structural protein production and improved VLP formation for HFMD.
Article Synopsis
- - It is commonly believed that the gene ORF1629 is crucial for baculovirus replication in insect cells, leading to the development of recombinant viruses through a baculovirus expression system.
- - Researchers discovered that a bacmid (a modified baculovirus) could still proliferate in insect cells even when it lacked a complete ORF1629, indicating that the gene is not strictly necessary for replication.
- - While ORF1629 is not essential, its absence resulted in slower growth rates and lower yields of the virus, along with larger but fewer occlusion bodies, affecting the overall production efficiency of baculoviruses like AcMNPV and BmNPV.
Article Synopsis
- The baculovirus expression system (BES) is effective for producing tough vertebrate proteins, and Ssp DnaB mini-intein serves as a beneficial fusion partner that can self-cleave under controlled conditions.
- Recombinant viruses were created to express multiple proteins fused to the mini-intein, resulting in successful intracellular cleavage during infection, although the timing varied with each protein.
- Overall, using Ssp DnaB mini-intein significantly boosted protein production, helping to counterbalance the loss from self-cleavage, and demonstrates its potential for easier purification and enhanced yields in BES applications.
Mamestra brassicae nucleopolyhedrovirus-K1 (MabrNPV-K1) was isolated from naturally infected M. brassicae (Lepidoptera: Noctuidae) larvae in Korea. The full genome sequences of MabrNPV-K1 were determined, analysed and compared to those of other baculoviruses.
View Article and Find Full Text PDFTo enhance the production efficiency of foreign protein in baculovirus expression systems, the effects of polyhedrin fragments were investigated by fusion expressing them with the enhanced green fluorescent protein (EGFP). Recombinant viruses were generated to express EGFP fused with polyhedrin fragments based on the previously reported minimal region for self-assembly and the KRKK nuclear localization signal (NLS). Fusion expressions with polyhedrin amino acids 19 to 110 and 32 to 110 lead to localization of recombinant protein into the nucleus and mediate its assembly.
View Article and Find Full Text PDFArticle Synopsis
- - Monochamus saltuarius is a pest that spreads pine wilt disease, causing significant harm to pine trees in Korea.
- - A fungus, identified as Beauveria bassiana and named MsW1, was isolated from dead M. saltuarius and showed potential as a biocontrol agent.
- - Virulence tests indicated that B. bassiana MsW1 resulted in 100% mortality of M. saltuarius adults and larvae after 11 and 13 days, respectively.