Application of the HPLC method for benzalkonium chloride determination in aerosol preparations.

Benzalkonium chloride (BAC) is a bacteriostatic agent used in the pharmaceutical industry as a preservative. BAC is a mixture of alkylbenzyldimethylammonium chlorides, the three most important of which being those with alkyl substituents C12, C14, C16 at the quaternary ammonium salt. The purpose of this study was to develop a method for determining benzalkonium chloride identity and content in aerosol preparations in which protein or steroid hormones are the active components.

Application of high-performance size exclusion chromatography to the determination of erythropoietin in pharmaceutical preparations.

High performance size exclusion chromatography with fluorimetric detection for the determination of erythropoietin in pharmaceutical preparations has been developed. The applied chromatographic system has been enabled a quick estimation of the identity and contents of the studied compound. The method was validated and showed good validation data in terms of linearity, precision and repeatability.

Determination of the content of desmopressin in pharmaceutical preparations by HPLC and validation of the method.

The aim of this study was to apply high performance liquid chromatography to the determination of content of desmopressin in pharmaceutical preparations and validation of the method. The satisfactory results have been obtained using a column Luna C 8.5 microm, 100 x 4.

New renin inhibitors with pseudodipeptidic units in P(1)-P(1') and P(2')-P(3') positions.

A series of four new potential renin inhibitors has been synthesized. The structure of the compounds was designed in such a way as to produce agents resistant to enzymatic degradation, metabolically stable, possibly potent and with improved oral absorption. All positions of the 8-13 fragment of the human angiotensinogen were occupied by unnatural units (two unnatural amino acids in positions P(3) and P(2) and two pseudodipeptides in positions P(1)-P(1') and P(2')-P(3')).