Production of Bacteriophages by Listeria Cells Entrapped in Organic Polymers.

Applications for bacteriophages as antimicrobial agents are increasing. The industrial use of these bacterial viruses requires the production of large amounts of suitable strictly lytic phages, particularly for food and agricultural applications. This work describes a new approach for phage production.

Foam-Mat Freeze-Drying of Bifidobacterium longum RO175: Viability and Refrigerated Storage Stability.

Foaming as a pretreatment was used prior to freeze-drying of Bifidobacterium longum RO175 to investigate the potential acceleration of the drying rate and increase in microorganism viability after the process. A study on storage of foamed and nonfoamed freeze-dried products at 4 °C completed this study. B.

Adhesion of Pathogenic Bacteria to Food Contact Surfaces: Influence of pH of Culture.

The adhesion of Aeromonas hydrophila, Escherichia coli O157:H7, Salmonella Enteritidis, and Staphylococcus aureus to hydrophobic and hydrophilic surfaces in cultures with different pHs (6, 7, and 8) was studied. The results indicated that the type of material had no effect on the attachment capacity of microorganisms, while environmental pH influenced the adhesion of A. hydrophila, E.

Effect of protective agents on the viability of geotrichum candidum during freeze-drying and storage.

The effect of 3 cryoprotective agents (trehalose, sucrose, and maltose) on the survival of concentrated cultures of Geotrichum candidum was studied. Initially, the effect of the carbohydrates at 9% and 23% concentrations or combined with skim milk (16%) was compared to the control (skim milk alone) immediately after freeze-drying. Two freeze-drying shelf temperatures, 25 degrees C and 35 degrees C, were investigated.

Probiotics reduce enterohemorrhagic Escherichia coli O157:H7- and enteropathogenic E. coli O127:H6-induced changes in polarized T84 epithelial cell monolayers by reducing bacterial adhesion and cytoskeletal rearrangements.

The aim of this study was to determine if probiotics reduce epithelial injury following exposure to Escherichia coli O157:H7 and E. coli O127:H6. The pretreatment of intestinal (T84) cells with lactic acid-producing bacteria reduced the pathogen-induced drop in transepithelial electrical resistance.

Iatrogenic paresthesia in the third division of the trigeminal nerve: 12 years of clinical experience.

Background: Iatrogenic paresthesia in the third division of the trigeminal nerve remains a complex clinical problem with major medicolegal implications. However, most lawsuits can be prevented through better planning of procedures and by obtaining informed consent. The purpose of this article is to present the authors" clinical experience over the past 12 years, to review the principles of prevention and management of trigeminal paresthesia and to highlight the resulting medicolegal implications.

Growth of Lactobacillus paracasei ssp. paracasei on tofu whey.

The liquid by-product of the soybean product tofu, tofu whey (TW), was used as a growth medium for the production of Lactobacillus paracasei ssp. paracasei LG3 cultures. The TW used in this study contained stachyose, raffinose, sucrose, fructose and glucose, but the strain used could only utilize the three latter.

Antiplatelet drugs : is there a surgical risk?

Acetylsalicylic acid has long been the only nonsteroidal anti-inflammatory drug recommended for the treatment and prevention of thromboembolic diseases. More recently, new compounds have been used in patients with vascular diseases. However, these drugs are often associated with longer bleeding times and greater operative risk.

Simple and Rapid Inhibition Enzyme Immunoassay for the Detection of Staphylococcal Enterotoxin B in Foods.

An absorption-inhibition ELISA (AI-ELISA) was developed to detect, within an hour, the staphylococcal enterotoxin B (SEB) in solutions and food homogenates. The SEB-containing sample is first incubated with a standardized concentration of antibody. The quantity of nonabsorbed anti-SEB Ab is evaluated by incubating a SEB-coated dipstick in the reaction mixture.

Attachment of Listeria monocytogenes to Stainless Steel, Glass, Polypropylene, and Rubber Surfaces After Short Contact Times.

This study was carried out to investigate the attachment capabilities of Listeria monocytogenes strain Scott A to stainless steel, glass, polypropylene, and rubber surfaces after short contact times at ambient (20°C) and cold storage temperatures (4°C) using scanning electron microscopy technique. Surface energy value of each surface was estimated by contact angle measurements. All surfaces displayed many possible harborages for L.

Production of Avid Rabbit Antibodies Against Staphylococcal Enterotoxins A and B.

The production of avid rabbit antibodies against staphylococcal enterotoxins A (SEA) and B (SEB) is described. The toxic effect of the SEA, measured by the weight loss of immunized rabbits, was greatly minimized by injecting the SEA-anti-SEA serum complex. The anti-SEA and anti-SEB antibody titers were estimated by a rapid ELISA on dipsticks (RD-ELISA) and allowed the selection of avid antibodies capable of reacting within 5 min with immobilized SEA or SEB.