Fetal umbilical Doppler in a population of 541 high-risk pregnancies: prediction of perinatal mortality and morbidity. Doppler Study Group.

Objective: To evaluate the usefulness of systematic umbilical Doppler in the assessment of high-risk pregnancies.

Method: In a prospective multicentre study, a group of high-risk pregnancies (intrauterine growth retardation, hypertension during pregnancy, abnormal obstetric history) was systematically studied by Doppler exploration of the fetal umbilical artery between 28 and 34 weeks. All the details of pregnancy development to the first postnatal days were collected and analysed a posteriori.

A murine monoclonal multireactive immunoglobulin kappa light chain.

N12.12 is a monoclonal immunoglobulin (Ig) kappa light chain (KLC) secreted by a B-cell hybridoma derived from spleen cells of a normal SJA mouse. No heavy chain was detected in the culture supernatant of this hybridoma using an enzyme immunoassay (EIA) and after polyacrylamide gel electrophoresis (SDS-PAGE) of the 35S-methionin biosynthetically labelled proteins secreted by the cells.

Thymic epithelium induces neither clonal deletion nor anergy to Mls 1a antigens.

Grafting of thymic anlagen from day-10 DBA/2 (H-2d; Mls-1a) embryos to newborn athymic BALB/c (H-2d; Mls-1b) mice leads to reconstitution of T cell populations in the recipients. Analysis of adult chimeras shows that their V beta T cell receptor (TcR) repertoires, particularly V beta 6 and V beta 8.1, do not significantly differ in most animals (10 out of 13) from those scored in control chimeras that received syngeneic thymic anlagen.

Transplantation tolerance correlates with high levels of T- and B-lymphocyte activity.

Mice tolerized (treated to make them tolerant) at birth to transplantation antigens by injection of semiallogeneic cells contain very high numbers of activated T and B lymphocytes in their spleen. Lymphoid hyperactivity correlates with the tolerant state: it is present only in animals accepting skin allografts. Tolerized mice that reject the allogeneic skin graft have approximately the same numbers of total and activated lymphocytes as normal mice.

Production and characterization of a mouse monoclonal antibody to the glycolipid asialo-GM1.

The glycosphingolipid asialo-GM1 (aGM1) is a true differentiation antigen of murine lymphoid cells. This glycolipid is highly immunogenic in the rabbit, but the antisera produced shows some cross reactivity with GM1, the naturally occurring sialylated derivative of aGM1. In the present study we examined the ability to raise anti-aGM1 antisera in the mouse.

Genetics of susceptibility to oral tolerance to ovalbumin.

Inbred mouse strains vary widely in their susceptibility to the induction of tolerance following oral (intragastric) administration of ovalbumin. Marked differences were found between strains that form a congenic pair differing at the H-2 complex: C3H/HeJ (H-2k) and C3H.SW (H-2b)-which were very susceptible and resistant to tolerance induction, respectively.