Publications by authors named "Jacquelyn R Roberts"
Phospholipids are the most abundant component in lipid membranes and are essential for the structural and functional integrity of the cell. In eukaryotic cells, phospholipids are primarily synthesized de novo through the Kennedy pathway that involves multiple enzymatic processes. The terminal reaction is mediated by a group of cytidine-5'-diphosphate (CDP)-choline /CDP-ethanolamine-phosphotransferases (CPT/EPT) that use 1,2-diacylglycerol (DAG) and CDP-choline or CDP-ethanolamine to produce phosphatidylcholine (PC) or phosphatidylethanolamine (PE) that are the main phospholipids in eukaryotic cells.
View Article and Find Full Text PDFArticle Synopsis
- The study focuses on the structural and proteomic analysis of Cag T4SSs derived from deletion mutants.
- It emphasizes the surprising structural independence between two key subdomains: the outer membrane complex (OMC) and the pilot region (PR).
- This finding challenges previous assumptions about the interdependence of these components within the T4SS structure.
Article Synopsis
- * The OMCC consists of a 14-fold symmetric outer membrane cap (OMC), a 17-fold symmetric periplasmic ring (PR), and an undefined stalk, with specific proteins (CagY, CagX, CagM, CagT) involved in organization.
- * Cryo-electron microscopy revealed that while the PR maintains structure without some proteins, the OMC’s organization requires multiple proteins, indicating a structural independence between the OMC and PR within the Cag T4SS
The induction of bulk autophagy by nitrogen starvation in baker's yeast (S. cerevisiae) involves the upregulation of many autophagy related proteins, including Atg7. One way to investigate the importance of this upregulation is to measure the size and number of autophagosomes formed when insufficient amounts of that protein are available.
View Article and Find Full Text PDF