Drought tolerance through biotechnology: improving translation from the laboratory to farmers' fields.

Water availability is a significant constraint to crop production, and increasing drought tolerance of crops is one step to gaining greater yield stability. Excellent progress has been made using models to identify pathways and genes that can be manipulated through biotechnology to improve drought tolerance. A current focus is on translation of results from models in controlled environments to crops in the field.

BROTHER OF LUX ARRHYTHMO is a component of the Arabidopsis circadian clock.

BROTHER OF LUX ARRHYTHMO (BOA) is a GARP family transcription factor in Arabidopsis thaliana and is regulated by circadian rhythms. Transgenic lines that constitutively overexpress BOA exhibit physiological and developmental changes, including delayed flowering time and increased vegetative growth under standard growing conditions. Arabidopsis circadian clock protein CIRCADIAN CLOCK ASSOCIATED1 (CCA1) binds to the evening element of the BOA promoter and negatively regulates its expression.

Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres.

Commercially improved crop performance under drought conditions has been challenging because of the complexity of the trait and the multitude of factors that influence yield. Here we report the results of a functional genomics approach that identified a transcription factor from the nuclear factor Y (NF-Y) family, AtNF-YB1, which acts through a previously undescribed mechanism to confer improved performance in Arabidopsis under drought conditions. An orthologous maize transcription factor, ZmNF-YB2, is shown to have an equivalent activity.