Evaluation of two VIDAS prototypes for detecting anti-HEV IgG.

Background: High performance anti-hepatitis E virus (HEV) IgG assays are crucial for epidemiology.

Objective: To evaluate the performance of 2 prototypes developed for the VIDAS automatic system for detecting anti-HEV IgG, one based on the ORF2 antigen (ORF2 prototype) and the other on the ORF2 and ORF3 antigens (ORF2/3 prototype), with reference to the Wantai anti-HEV IgG assay.

Study Design: The sensitivity of each assay was determined by testing 113 blood samples, 63 from immunocompetent and 50 from immunocompromised patients, with a proven HEV infection defined by detecting HEV RNA.