Cell Boundary Confinement Sets the Size and Position of the E. coli Chromosome.

Although the spatiotemporal structure of the genome is crucial to its biological function, many basic questions remain unanswered on the morphology and segregation of chromosomes. Here, we experimentally show in Escherichia coli that spatial confinement plays a dominant role in determining both the chromosome size and position. In non-dividing cells with lengths increased to 10 times normal, single chromosomes are observed to expand > 4-fold in size.

Bacterial Chemoreceptor Imaging at High Spatiotemporal Resolution Using Photoconvertible Fluorescent Proteins.

We describe two methods for high-resolution fluorescence imaging of the positioning and mobility of E. coli chemoreceptors fused to photoconvertible fluorescent proteins. Chemoreceptors such as Tar and Tsr are transmembrane proteins expressed at high levels (thousands of copies per cell).

Super-resolution imaging of light-matter interactions near single semiconductor nanowires.

Nanophotonics is becoming invaluable for an expanding range of applications, from controlling the spontaneous emission rate and the directionality of quantum emitters, to reducing material requirements of solar cells by an order of magnitude. These effects are highly dependent on the near field of the nanostructure, which constitutes the evanescent fields from propagating and resonant localized modes. Although the interactions between quantum emitters and nanophotonic structures are increasingly well understood theoretically, directly imaging these interactions experimentally remains challenging.

Assessment of resuspended matter and redistribution of macronutrient elements produced by boat disturbance in a eutrophic lagoon.

Harvesting of macroalgae by specially equipped boats in a shallow eutrophic lagoon produces evident sediment resuspension. To outline the environmental effects of this disturbance, we examined the quantity of fall-out and the distances travelled by sediment and macronutrients from the source of boat disturbance. Resuspended sediment fall-out (RSFO) was trapped at different distances from the boat path to determine total dry weight, total nitrogen (TN), total carbon (TC), total organic carbon (TOC), total sulphur (TS) and total phosphorus (TP).