In vitro evaluation of a hybrid negative pressure system for wound therapy.

Objective: The objective of this study was to assess whether negative pressure could be maintained accurately and repeatably with a wall-suction-based hybrid negative pressure wound therapy (h-NPWT) system by comparing it with a commercial negative pressure wound therapy (NPWT) device.

Study Design: In vitro experimental study.

Methods: A commercial NPWT device (control) and three h-NPWT devices, with 0, 3, and 6 meters of additional tubing using the hospital-wall suction (groups 1, 2, and 3 respectively), were applied sequentially to a commercial NPWT dressing on a silicone skin substrate and set to run at a continuous pressure of -125 mmHg.

Retrospective Comparison of Titanium Hybrid Locking Plate with Stainless Steel Hybrid Dynamic Compression Plate for Pancarpal Arthrodesis: 23 Dogs.

Objective:  The aim of this study was to evaluate outcomes and complications of dogs that had pancarpal arthrodesis (PCA) using a titanium hybrid advanced locking plate system (ALPS) and stainless steel hybrid dynamic compression plate (HDCP).

Study Design:  Retrospective observational study.

Methods:  Medical records (2007-2020) were reviewed for cases that had PCA performed using ALPS or HDCP.

Minicircle Mediated Gene Delivery to Canine and Equine Mesenchymal Stem Cells.

Gene-directed tissue repair offers the clinician, human or veterinary, the chance to enhance cartilage regeneration and repair at a molecular level. Non-viral plasmid vectors have key biosafety advantages over viral vector systems for regenerative therapies due to their episomal integration however, conventional non-viral vectors can suffer from low transfection efficiency. Our objective was to identify and validate in vitro a novel non-viral gene expression vector that could be utilized for ex vivo and in vivo delivery to stromal-derived mesenchymal stem cells (MSCs).

Suppression of dedifferentiation and hypertrophy in canine chondrocytes through lentiviral vector expression of Sox9 and induced pluripotency stem cell factors.

Objectives: Prolonged in vitro culture of primary articular chondrocytes results in dedifferentiation to a fibroblast-like cell with reduced expression of the Sox9 transcription factor and the extracellular matrix protein collagen II. The ability to genetically-modify chondrocytes to allow both proliferation and maintenance of an articular phenotype may provide increased numbers of appropriate cells for regeneration of large cartilage defects.

Results: Canine chondrocytes were expanded in monolayer culture and transduced with a lentiviral vector expressing Sox9 or in combination with a multicistronic lentiviral vector expressing the four induced pluripotency stem (iPS) cell factors, Oct4, Klf4, Sox2 and c-Myc (OSKM).

Canine elbow dysplasia: aetiopathogenesis and current treatment recommendations.

Elbow dysplasia is a common debilitating condition of large and giant breed dogs. Environmental factors and a complex genetic heritability play a role in predisposing dogs to elbow dysplasia with two aetiopathogeneses suggested for the development of the disease. Osteochondrosis was initially thought to cause elbow dysplasia, but more recent evidence has strongly supported various forms of joint incongruity as the most likely cause in most cases.

Conditioned response to a magnetic anomaly in the Pekin duck (Anas platyrhynchos domestica) involves the trigeminal nerve.

There have been recent calls to develop protocols that collect unambiguous measures of behaviour using automatic techniques in conditioning experiments on magnetic orientation. Here, we describe an automated technique for recording the behaviour of Pekin ducks in a conditioning test that allows them to express unrestricted searching behaviour. Pekin ducks were trained to find hidden food in one corner of a square arena below which was placed a magnetic coil that produced a local magnetic anomaly.