Complete genome of a rare subsp. serovar Hessarek from human stool.

We present a complete genome of subsp. serovar Hessarek isolated from a human stool from an outbreak linked to egg consumption in South Australia. Orientation of the operon and characteristics of the virulence plasmid indicates that this serovar is virulent toward humans and birds.

An open-source dual-beam spectrophotometer for citizen-science-based water quality monitoring.

Efforts to understand and mediate threats to water supplies rely on collection of reliable data at large scale, a goal which is often limited by availability of tools that are both affordable and reliable. We present here a low-cost, easy-to-use, do-it-yourself (DIY) spectrometer for measurement of a variety of relevant solute concentrations when coupled with inexpensive commercially-available reagents. Comparison of its performance with commercial options demonstrates the potential value of this device as transparent, versatile, and accurate-enough alternative for widespread application.

Evaluation of in vivo bioactivities of recombinant hypo- (FSH) and fully- (FSH) glycosylated human FSH glycoforms in Fshb null mice.

The hormone - specific FSHβ subunit of the human FSH heterodimer consists of N-linked glycans at Asn and Asn residues that are co-translationally attached early during subunit biosynthesis. Differences in the number of N-glycans (none, one or two) on the human FSHβ subunit contribute to macroheterogeneity in the FSH heterodimer. The resulting FSH glycoforms are termed hypo-glycosylated (FSH, missing either an Asn or Asn N-glycan chain on the β - subunit, respectively) or fully glycosylated (FSH, possessing of both Asn and Asn N-linked glycans on the β - subunit) FSH.