Genetic Characterization of Antimicrobial-Resistant Isolated from a Mixed-Use Watershed in Northeast Georgia, USA.

In order to determine the role of surface water in the development and spread of antibiotic-resistant (AR) bacteria, water samples were collected quarterly from 2015 to 2016 from a mixed-use watershed in Georgia. In our previous study, 496 were isolated from surface water, out of which, 34 isolates were resistant to antimicrobials. For the current study, these 34 AR were characterized using pulsed-field gel electrophoresis, AR gene detection, plasmid replicon typing, class I integron detection, and multi-locus sequence typing.

Rapid and Stable Microbial Community Assembly in the Headwaters of a Third-Order Stream.

Small streams and their headwaters are key sources of microbial diversity in fluvial systems and serve as an entry point for bacteria from surrounding environments. Community assembly processes occurring in these streams shape downstream population structure and nutrient cycles. To elucidate the development and stability of microbial communities along the length of a first- through third-order stream, fine-scale temporal and spatial sampling regimes were employed along McNutt Creek in Athens, GA, USA.