Spironolactone affects cardiovascular and craniofacial development in zebrafish embryos (Danio rerio).

Spironolactone, a potassium-sparing diuretic and aldosterone antagonist, is a mineralocorticoid hormone commonly prescribed to patients suffering from heart failure, hirsutism, dermatological afflictions, and hypertension. Interestingly, relatively little work has been done on the development of vertebrate embryos after exposure to this compound. Here, we treat zebrafish embryos with spironolactone at 10 M, 10 M, or 10 M, and observe them after three to seven days of exposure.