Publications by authors named "Jaclyn B Dunne"
Article Synopsis
- Recent advancements in single-cell imaging have enhanced our understanding of how individual cells interact and influence disease progression.
- The described protocol utilizes MALDI-IHC with UV photocleavable mass tags to enable the simultaneous imaging of different cell types.
- This method integrates N-glycomic and ECM-targeted proteomic imaging from the same tissue section, offering a holistic view of the cellular and extracellular microenvironments.
Polyploid cells contain more than two copies of each chromosome. Polyploidy has important roles in development, evolution, and tissue regeneration/repair, and can arise as a programmed polyploidization event or be triggered by stress. Cancer cells are often polyploid.
View Article and Find Full Text PDFPolyploid cells contain more than two copies of each chromosome. Polyploidy has important roles in development, evolution, and tissue regeneration/repair, and can arise as a programmed polyploidization event or be triggered by stress. Cancer cells are often polyploid.
View Article and Find Full Text PDFMatrix assisted laser desorption/ionization imaging has greatly improved our understanding of spatial biology, however a robust bioinformatic pipeline for data analysis is lacking. Here, we demonstrate the application of high-dimensionality reduction/spatial clustering and histopathological annotation of matrix assisted laser desorption/ionization imaging datasets to assess tissue metabolic heterogeneity in human lung diseases. Using metabolic features identified from this pipeline, we hypothesize that metabolic channeling between glycogen and N-linked glycans is a critical metabolic process favoring pulmonary fibrosis progression.
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