Antenatal betamethasone depresses maternal and fetal aldosterone levels.

Antenatal glucocorticoids are used to mature lung function in fetuses at risk for preterm delivery, but they also suppress cortisol synthesis in both pregnant women and their fetuses. We recently discovered in pregnant rabbits that even though exogenous betamethasone is not a mineralocorticoid, it also suppresses production of aldosterone. Lower aldosterone levels were linked to reduced P450 side chain cleavage(P450scc) messenger RNA levels in the rabbit maternal and fetal adrenal cortex.

Pregnancy-specific changes in uterine artery endothelial cell signaling in vivo are both programmed and retained in primary culture.

Ovine uterine artery (UA) endothelial cells (UAEC) maintained in culture to passage 4 retain pregnancy-specific changes in vasodilator production, which in turn is associated with differences in Ca(2+) and ERK 1/2 signaling. The question remains whether this is an accurate portrayal of the situation in vivo, or more simply whether these same signaling responses seen at passage 4 accurately reflect those functioning in the cells in vivo. Small groups of endothelial nitric oxide synthase-positive cells from both pregnant and nonpregnant ewes were freshly isolated and used to image changes in the intracellular free calcium concentration ([Ca(2+)](i)) using fura 2 and to detect ERK 1/2 phosphorylation by immunocytochemistry.