Immunogenicity of a recombinant chimera composed of CROP domain segments from the hemorrhagic and lethal toxins of Paeniclostridium sordellii.

Paeniclostridium sordellii is responsible for severe infections in horses, cattle, and sheep; however, conventional vaccines exhibit limitations in production and immunogenicity. This study assessed the immunogenicity of a recombinant bacterin composed of a chimera (rQTcsHL) that combines segments from the lethal (TcsL) and hemorrhagic (TcsH) toxins in mice and sheep. Both immunized animal groups exhibited elevated levels of IgG, with the mice demonstrating moderate protection (<50%) against lethal challenges, comparable to that of the conventional vaccine.

Fiberglass posts reduced fractures in endodontically treated teeth restored with veneers: a case-control study.

This study aimed to evaluate the effects of fiberglass posts on fracture behavior in endodontically treated teeth (ETT) restored with composite veneers. Adult patients who had endodontically treated anterior teeth restored with composite veneers, with or without fiberglass posts, were divided into the case (with fractured teeth) and control (without fractured teeth) groups. All fracture patterns were sorted by a 6-point scoring system: 0, no fracture; 1, veneer buccal fracture; 2, incisal edge fracture; 3, coronal middle-third fracture; 4, coronal cervical fracture; 5, coronal and root fracture; and 6, root fracture.

The Therapeutic Potential of Physical Exercise in Cancer: The Role of Chemokines.

The global increase in cancer cases and mortality has been associated with inflammatory processes, in which chemokines play crucial roles. These molecules, a subfamily of cytokines, are essential for the migration, adhesion, interaction, and positioning of immune cells throughout the body. Chemokines primarily originate in response to pathogenic stimuli and inflammatory cytokines.

Probiotic Microorganisms in Inflammatory Bowel Diseases: Live Biotherapeutics as Food.

(1) Background: Inflammatory bowel diseases (IBDs) are characterized by chronic and complex inflammatory processes of the digestive tract that evolve with frequent relapses and manifest at any age; they predominantly affect young individuals. Diet plays a direct role in maintaining the gut mucosal integrity and immune function. Regarding the diet, the administration of probiotics stands out.

A Study of Glabellar Contraction Patterns in African Descendants.

Background: Botulinum toxin is a well-established treatment for dynamic glabellar lines. Glabellar contraction patterns were described previously in the general Brazilian population and also among Koreans, Chinese, and Indian individuals. So far, no study has addressed glabellar contraction "patterns" in Black subjects.

Design of Natterins-based peptides improves antimicrobial and antiviral activities.

The biochemical analysis of animal venoms has been intensifying over the years, enabling the prediction of new molecules derived from toxins, harnessing the therapeutic potential of these molecules. From the venom of the fish , using methods for predicting antimicrobial and cell-penetrating peptides, two peptides from Natterins with promising characteristics were synthesized and subjected to and analysis. The peptides were subjected to stability tests and antimicrobial assays, cytotoxicity in murine fibroblast cells, antiviral assays against the Chikungunya virus, and the toxicity on was also evaluated.

Native and Non-Native Soil and Endophytic spp. from Semi-Arid Sisal Fields of Brazil Are Potential Biocontrol Agents for Sisal Bole Rot Disease.

Sisal () bole rot caused by is the main phytosanitary problem affecting sisal in the Brazilian semi-arid region. The aim of this study was to evaluate spp. as biocontrol agents for sisal bole rot.

An extracellular vesicle delivery platform based on the PTTG1IP protein.

Extracellular vesicles (EVs) are promising therapeutic delivery vehicles, although their potential is limited by a lack of efficient engineering strategies to enhance loading and functional cargo delivery. Using an in-house bioinformatics analysis, we identified N-glycosylation as a putative EV-sorting feature. PTTG1IP (a small, N-glycosylated, single-spanning transmembrane protein) was found to be a suitable scaffold for EV loading of therapeutic cargoes, with loading dependent on its N-glycosylation at two arginine residues.

In Vivo Anti-Inflammatory Activity of D-Limonene in a Rat Model of Monocrotaline-Induced Pulmonary Hypertension: Implications to the Heart Function.

Background: D-limonene (D-L) is the major monocyclic monoterpene in citrus plants with anti-inflammatory properties. Pulmonary hypertension (PH) can cause right heart dysfunction and increases the risk of death, partially due to inflammatory response in the heart.

Objective: To evaluate the possible protective effect of D-L on cardiac function in a rat model of monocrotaline-induced PH (MCT-PH).

Repercussions of the pandemic on tuberculosis control actions from the perspective of health professionals.

Objectives: to analyze the repercussions of the COVID-19 pandemic on tuberculosis control actions from the perspective of primary health care professionals.

Methods: this descriptive study with a qualitative approach was conducted from November 2022 to April 2023, using semi-structured interviews with 11 key informant professionals from primary health care units in a Brazilian capital. Data were organized using Atlas.

Transcriptional Regulation of the Human MGP Promoter: Identification of Downstream Repressors.

Matrix Gla protein (MGP) is a vitamin K-dependent γ-carboxylated protein that was initially identified as a physiological inhibitor of ectopic calcification, primarily affecting cartilage and the vascular system. Mutations in the gene were found to be responsible for the Keutel syndrome, a condition characterized by abnormal calcifications in the cartilage, lungs, brain, and vascular system. has been shown to be dysregulated in several tumors, including cervical, ovarian, urogenital, and breast cancers.

Morbidity associated with schistosomiasis in adult population of Chókwè district, Mozambique.

Background: Mozambique is one of the countries with the highest prevalence of schistosomiasis, although there is little data on the prevalence of disease and associated morbidity in the adult population. This study aimed to describe and characterize the morbidity associated with schistosomiasis in the adult population of Chókwè district and to explore the use of anamnestic questionnaires and urine dipsticks, as well as point-of-care ultrasound for urinary related findings, to better characterize disease prevalence and morbidity.

Methodology: Between April and October 2018, we conducted a cross-sectional study embedded within the Chókwè Health Research and Training Centre.

Unilateral Ptosis in a 72-Year-Old Male: Early Diagnosis of Myasthenia Gravis Using the Ice Pack Test.

Ptosis, or eyelid drooping, can be an early indicator of various neurological and muscular disorders, underscoring the need for accurate diagnosis to ensure proper management. Myasthenia gravis (MG), a neuromuscular disorder, may initially present with isolated ocular symptoms, often resulting in diagnostic delays. This report details the case of a 72-year-old male who presented to the emergency department with a two-week history of progressive unilateral ptosis.

Brakes and leverages for reducing the environmental impact of laboratory animal facilities: A case study at the Institut Pasteur.

The Institut Pasteur has set the ambition to encourage all staff to get involved in sustainable development across all departments on campus. The animal facility staff joined the efforts of the sustainable development department to analyse current and future processes and identify potential solutions and related brakes and leverages for the reduction of the animal facilities' environmental impact. The first step was to collect the managers' experience on the local initiatives.

Macaiba palm pulp (Acrocomia intumescens Drude) improves memory and induces anxiolytic-like behavior in dyslipidemic rats.

Macaiba pulp is a source of bioactive compounds. This study aimed to evaluate the effects of macaiba pulp on anxiety behavior, memory and brain oxidative stress in dyslipidemic rats. The animals were divided into four groups (n = 10): Control (CG), Macaíba (MG), Dyslipidemic (DG) and Dyslipidemic Macaiba (DMG).

Sulfur fertilization and inoculation of soybean with Azospirillum brasilense and Bradyrhizobium spp. can improve grain yield and quality.

The success of biological nitrogen fixation (BNF) in soybean depends on two crucial factors, viz., seed inoculation and soil nutritional balance. The macronutrient sulfur (S) is vital to the formation of ferredoxin, a common source of electrons that controls the proper functioning of the subunits of the enzyme nitrogenase, responsible for the conversion of atmospheric nitrogen (N) to ammonia (NH).

Marchiafava-Bignami Disease: A Case Report of a Reversible Cause of Dementia.

Marchiafava-Bignami disease (MBD) is a rare neurological disorder predominantly associated with chronic alcohol use, characterized by demyelination and necrosis of the corpus callosum. The condition often presents with cognitive dysfunction, motor deficits, and altered consciousness, which can range from mild confusion to severe stupor. Early recognition and treatment are crucial for improving outcomes.

High-intensity interval training versus moderate-intensity continuous training on exercise capacity and health-related quality of life in patients with coronary artery disease: An updated systematic review and meta-analysis.

Background: Despite the well-known positive effects of exercise in patients with coronary artery disease, the best exercise training protocol is still under discussion.

Objective: We performed a systematic review and a meta-analysis to investigate the effects of high-intensity interval training (HIIT) versus moderate-intensity continuous training (MICT) on exercise capacity and health-related quality of life (HRQoL) in patients with coronary artery disease.

Methods: We searched MEDLINE/PubMed, the Cochrane Library EMBASE, and the PEDro database for randomized controlled trials that evaluated the effects of HIIT versus MICT.

High-Throughput Single-Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC).

Extracellular matrix (ECM) guides cell behavior and tissue fate. Cell populations are notoriously heterogeneous leading to large variations in cell behavior at the single-cell level. Although insights into population heterogeneity are valuable for fundamental biology, regenerative medicine, and drug testing, current ECM analysis techniques only provide either averaged population-level data or single-cell data from a limited number of cells.

Anti-EGFR Rechallenge in Metastatic Colorectal Cancer and the Role of ctDNA: A Systematic Review and Meta-analysis.

Background: Metastatic colorectal cancer (mCRC) remains a significant clinical challenge. While anti-EGFR inhibitors have improved survival rates, their long-term efficacy is limited by disease progression, which is often associated with the development of acquired resistance mutations. However, some patients may regain sensitivity to anti-EGFR agents after alternative therapies, suggesting a potential benefit for rechallenge strategies.

High-Intensity Interval Training for Cancer Patients: A Review of Key Considerations for Exercise Prescription.

Background: High-intensity interval training (HIIT) performed before, during, and after cancer treatment can attenuate the adverse effects induced by anti-cancer drugs. A clear presentation and rationale of characteristics of HIIT variables is vital to produce the expected HIIT adaptations in cancer patients. However, there are concerns regarding the HIIT protocols used in the cancer literature.

Benzocarbazoledinones as SARS-CoV-2 Replication Inhibitors: Synthesis, Cell-Based Studies, Enzyme Inhibition, Molecular Modeling, and Pharmacokinetics Insights.

Endemic and pandemic viruses represent significant public health challenges, leading to substantial morbidity and mortality over time. The COVID-19 pandemic has underscored the urgent need for the development and discovery of new, potent antiviral agents. In this study, we present the synthesis and anti-SARS-CoV-2 activity of a series of benzocarbazoledinones, assessed using cell-based screening assays.

Risk Factors for Non-Communicable Diseases in Refugees, Asylum Seekers, and Subsidiary Protection Beneficiaries Resettled or Relocated in Portugal Between 2015 and 2020.

Non-communicable diseases, previously thought of as a problem of high-income countries, now coexist in low- and middle-income countries, including the countries of origin for many refugees traveling to Europe. We aimed to describe the prevalence of risk factors for non-communicable diseases among refugees, asylum seekers, and subsidiary protection beneficiaries resettled or relocated in Portugal between 2015 and 2020 and compare these to the prevalence of risk factors in the 12 months before they left their country of origin. A cross-sectional study was conducted between 2019 and 2020 of all refugees, asylum seekers, and subsidiary protection beneficiaries attending a Lisbon, Portugal refugee center.