Hidden life cycle stages of the trypanosomatid Blastocrithidia triatomae Cerisola et al. 1971 (Protista: Excavata: Kinetoplastea) unveiled by a new cultivation method.

Blastocrithidia triatomae is a monoxenic trypanosomatid parasite of triatomines, sharing the same insect vectors with Trypanosoma cruzi Chagas, 1909 and T. rangeli Tejera, 1920. It is known to cause a complex syndrome in insects which induces severe metabolic disorders and increasing in mortality rates.

Relationship among Dietary Intake of Vitamin E, Lipid Peroxidation Markers, and C-Reactive Protein in Flu-Like Patients Diagnosed with COVID-19.

Objective: This research aimed to assess the intake of vitamin E and its relationship with lipid peroxidation markers and C-reactive protein levels in patients with flu symptoms and COVID-19 diagnosis.

Methods: A cross-sectional study with 121 patients of both sexes assisted at two basic health units in the city of Teresina, Piauí, with COVID-19 diagnosis confirmed through real-time reverse transcription polymerase chain reaction, was performed between the 3 and 7 days of flu symptoms. The global nutritional status and the measurement of waist circumference were assessed according to the World Health Organization recommendations.

Morphological analysis and lipid composition of the stable fly Stomoxys calcitrans salivary glands reinforce important features of feeding behaviour.

Stomoxys calcitrans L. (Diptera: Muscidae), the stable fly, is a hematophagous insect of great veterinary importance, because it is a mechanical vector of diverse pathogens in livestock. The saliva of blood-feeding insects presents important pharmacologically active molecules that impair blood clotting, promote vasodilation and modulate the host immune system response, crucial processes for successful feeding.

A molecular and morphological study of Ascaris suum in a human-pig contact scenario in northeastern Brazil.

The aim of the present study was to assess morphologic and genetic data on ascariasis in swine (Sus scrofa domesticus) and humans in low-resource rural and periurban communities in the state of Piauí, Brazil. Our cross-sectional survey included 100 fecal samples obtained from swine and 682 samples from humans. Fifteen pigs were necropsied.

Study on Morphological Changes and Interference in the Development of Caused by Some Essential Oil Constituents.

Dengue, Chikungunya and Zika are arboviruses, transmitted by the mosquito , that cause high mortality and serious health consequences in human populations. Efforts to control are important for preventing outbreaks of these diseases. Essential oil constituents are known to exhibit many activities, such as their use as larvicides.

Association between the potential distribution of Lutzomyia longipalpis and Nyssomyia whitmani and leishmaniasis incidence in Piauí State, Brazil.

Background: Leishmaniases are vector borne diseases caused by Leishmania spp. parasites transmitted by female sandflies (Diptera: Psychodidae) whose geographic distribution is influenced by environmental factors. Among the main tools for studying the distribution of vector species, modeling techniques are used to analyze the influence of climatic and environmental factors on the distribution of these insects and their association with human cases of the disease.

Characterization of External Female Genitalia in Five Laporte Species of South America (Hemiptera: Reduviidae: Triatominae).

Currently, there are 158 valid species of triatomines, all of which are potential vectors of , the etiological agent of Chagas disease. The correct taxonomic identification of triatomines is essential since each species hos a different epidemiological importance. The aim of the study is to compare five species of South American .

A Novel Model of Pathogenesis of Propagules through the Midguts of Larvae.

We assessed the effect of the entomopathogenic fungus against . Conidia of strains CG 489, CG 153, and IBCB 481 were grown in Adamek medium under different conditions to improve blastospore production. Mosquito larvae were exposed to blastospores or conidia of the three fungal strains at 1 × 10 propagules mL.

Monthly abundance and diversity of mosquitoes (Diptera: Culicidae) in an Atlantic Forest area of Rio de Janeiro, Brazil.

Several mosquito species in the Atlantic Forest are yellow fever vectors; therefore, this biome can represent a potential risk to the human population. Studies on mosquitoes from predominantly sylvatic areas produce valuable data for understanding the emergence of new epidemics. In addition, they can elucidate environmental components favoring or hindering biodiversity and species distribution.

Entomopathogenic fungi and Schinus molle essential oil: The combination of two eco-friendly agents against Aedes aegypti larvae.

Aedes aegypti transmits arbovirus, which is a public health concern. Certain filamentous fungi have the potential to control the disease. Here, the effects of Metarhizium anisopliae s.

Spawning behavior of Aedini (Diptera: Culicidae) in a remnant of Atlantic Forest in the state of Rio de Janeiro.

Background: Mosquito assemblages are organized along an ecological gradient, including small habitats where interspecific competition predominates and large permanent habitats where predation predominates. This study aimed to analyze the oviposition behavior of mosquitoes regarding the preference for traps installed at two different heights with regard to ground level and the tendency to share spawning sites in an Atlantic Forest fragment in Nova Iguaçu, State of Rio de Janeiro, Brazil.

Methods: The eggs were collected from April 2018 to March 2019.

Bionomy of Mosquitoes in Bamboo Internodes in an Atlantic Forest Remnant of the State of Rio De Janeiro, Brazil.

Living bamboo stalks are one of the most specialized habitats for mosquito oviposition and immature development. Most of the mosquito species that breed in these habitats are sylvatic, and some are of importance for public health as possible vectors of pathogens. Perforated internodes are a very specialized environment due to the difficulty of access.

Evaluation of the Diversity of Culicidae Vectors of the Sylvatic Yellow Fever Virus in Atlantic Forest Remnants with the Use of Ovitraps and Bamboo Traps.

Mosquitoes transmit several pathogens in tropical regions, especially in forest areas. The diseases caused by these pathogens include malaria and several arboviruses such as yellow fever. Brazil has the largest endemic area in the world for yellow fever.

Larvicidal activity, route of interaction and ultrastructural changes in Aedes aegypti exposed to entomopathogenic fungi.

Blastospores or conidia (formulated or not) of entomopathogenic fungi were assessed against Aedes aegypti larvae. Larvae (L) were exposed to 10, 10, 10, and 10 propagules mL water suspension. Mineral oil at 0.

External female genitalia of Triatoma jatai, Triatoma costalimai and Triatoma williami (Hemiptera: Reduviidae: Triatominae).

Background: Taxonomic identification of triatomines is generally performed based on aspects of their external morphology. However, the use of a multidisciplinary approach, considering morphological aspects of the external genitalia, morphometry, genetics, and phylogeography has been suggested, especially for similar and/or cryptic species. The rupestral species Triatoma jatai Gonçalves et al.

Spatial distribution of synanthropic triatomines in Piaui State, Northeastern Brazil.

This study aimed to describe the spatial distribution and assess entomological indicators of synanthropic triatomines in Piaui State, Northeastern Brazil. We used surveillance data on the detection, identification and assessment of natural infection with trypanosomatids from triatomines in the State from 2014 to 2017. The State was divided into four macroregions.

Ultrastructure and morphometry of the egg of Coquillettidia albifera (Prado) with illustrations of male genitalia (Diptera: Culicidae).

The ultrastructure and morphometry of the egg of Coquillettidia albifera (Prado) (Diptera: Culicidae) studied under scanning electronic microscopy is presented for the first time. The identification of the species was confirmed by examination of the genitalia of males collected with females. The male genitalia are redescribed and digital photographs are provided.

Scanning Electron Microscopy of Sand Flies of the Chagasi Series, Psychodopygus (Diptera: Psychodidae) Genus, Focusing on the Genitalia.

Males of cryptic or closely related species present great morphological variation in their genitalia, whereas females, such as those of the Chagasi Series of the Psychodopygus Mangabeira, 1941 genus, are more similar. Therefore, our aim was to study the fine structure of the male genitalia of five species of the Chagasi Series to better understand the variation in their morphology and its influence on the copulatory process. The sand fly species were captured in the following Brazilian states: Psychodopygus chagasi (Costa Lima, 1941) (Rondônia), Psychodopygus complexus (Mangabeira, 1941) (Tocantins), Psychodopygus squamiventris maripaensis (Floch & Abonnenc, 1946) (Amapá), Psychodopygus squamiventris squamiventris (Lutz & Neiva, 1912) (Amazonas), and Psychodopygus wellcomei Fraiha, Shaw & Lainson, 1971 (Pará and Ceará).

Triatomine dispersion rates and their association with socioeconomic and environmental conditions in Northeastern Brazil, from 2009 to 2013.

Triatomines are vectors of Trypanosoma cruzi , the etiological agent of Chagas disease, which affects between 6 and 12 million people in Latin America, with an incidence rate of 12 thousand cases per year. In the Ceara State, the predominance of the caatinga biome, coupled with a large rural area with precarious human habitations, provides several s shelters for these insects. In this study, we determined the spatiotemporal distribution of triatomine dispersion rates in the Cariri region, Southern Ceara and ascertained the possible association between these rates with socioeconomic and environmental factors.

Naturally Infected by : A Public Health Concern.

The rupestrian species has been found infected by in wild, peridomicile, and intradomicile environments in the municipality of Aurora do Tocantins, Tocantins, Brazil. Proximity between rock outcrops increases the risk of vector transmission of Chagas disease via this species. This work describes a focus of colonization by specimens infected by in rock outcrops located in an urban area in this municipality.

Chagasic infection among blood donors in Brazil: an integrative review.

Based on the literature, this work aimed to discuss infection by Chagas disease among blood donors in Brazil. Studies on the prevalence of infection in donors or candidates for blood donation in Brazil are important. The prevalence of infection appears to be a sensitive indicator and can be a true marker of the risk of the transmission of Chagas disease by blood transfusion.

Entomological survey in the state of Piauí, Northeastern Brazil, reveals intradomiciliary colonization of Triatoma brasiliensis macromelasoma.

This survey aimed to assess the presence of triatomine vectors of Chagas disease within the rural communities of São João do Piauí, Northeast Brazil. Intradomiciliary and peridomiciliary collection strategies were implemented wherein 279 specimens of Triatoma brasiliensis macromelasoma, both nymph and adult were found in 15 (50%) of the studied homes. Of the intradomiciliary insects, 73 (67.

Laboratory evaluation of Brevibacillus laterosporus strains as biocidal agents against Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) larvae.

The biocidal activity of three strains of Brevibacillus laterosporus upon the post-embryonic developmental stages of Chrysomya megacephala was evaluated. Bioassays were performed to verify lethal and sub-lethal effects including ultra-structural changes in the midgut. Among the strains assayed, Shi3 presented the highest larval mortality rates, achieving 70% at a concentration of 1×10 spores/g of diet.

New sex-determination system in the genus Panstrongylus (Hemiptera: Reduviidae) revealed by chromosomal analysis of Panstrongylus lutzi.

Background: Panstrongylus lutzi (Neiva & Pinto, 1923) is a triatomine species native to Caatinga habitats in north-eastern Brazil. It is considered an important vector of Chagas disease in this region, presenting high rates of natural infection with Trypanosoma cruzi Chagas, 1909, and readily invading houses by flight. This study describes a previously unknown chromosomal sex system in the genus Panstrongylus based on P.