Diabetes and corneal cell densities in humans by in vivo confocal microscopy.

Purpose: Diabetes is accompanied by an increased autofluorescence of the cornea, probably because of accumulation of advanced glycation end products (AGEs). The pathogenic mechanism is still unknown. This study aimed to quantify differences in corneal cell densities between diabetic patients and healthy controls.

Corneal cell density measurement in vivo by scanning slit confocal microscopy: method and validation.

Purpose: Method and validation of a technique to quantify cell density in vivo in 6 corneal layers with a scanning slit confocal microscope (SSCM).

Method: A confocal image of a small volume in a corneal layer is registered on videotape. Cells or nuclei according to a layer classification are counted manually using an unbiased frame.

Lack of fluorophotometric evidence of aqueous-vitreous barrier disruption after posterior capsulorhexis.

Purpose: To evaluate the integrity of the aqueous-vitreous barrier by assessing the flow of fluorescein from the anterior chamber to the anterior vitreous using fluorophotometry in eyes with a posterior continuous curvilinear capsulorhexis (PCCC) and in eyes without a PCCC.

Setting: University Hospital Antwerp, Edegem, Belgium.

Methods: Ten patients had bilateral extracapsular cataract extraction with implantation of an intraocular lens.

Corneal autofluorescence in relation to permeability of the blood-aqueous barrier in diabetic patients with clinically significant macular edema and in an age-matched control group.

Purpose: Corneal autofluorescence is related to advanced glycation end products formed by glucose that reaches the cornea via the aqueous humour. The aim of the study was to examine the influence on autofluorescence of changes in permeability of the blood aqueous barrier.

Methods: Corneal autofluorescence was measured in 50 diabetic patients with clinically significant macular edema and in 28 age-matched control subjects.

Derivation of lenticular transmittance from fluorophotometry.

Purpose: To derive transmittance spectra for the human lens using the ratio between posterior and anterior autofluorescence of the lens as measured by fluorophotometry.

Methods: Transmittance spectra of the lens can be described with a one-parameter model to a high degree of accuracy. The parameter m of this model defines the differences between lens transmittance spectra of individuals.

Reflex and steady state tears in patients with latent stromal herpetic keratitis.

Purpose: To compare tear production in patients with stromal herpetic keratitis with that in healthy control subjects.

Methods: After instillation of 2 microL fluorescein into both eyes, the tear-fluorescein concentration was measured by fluorophotometry. During the first 10 minutes, steady state tear turnover (TTO-1) was determined.