[Protective effects of 3, 6-dimethamidodibenzopyriodinium citrate on myocardial injury induced by ischemia and reperfusion in rats].

3, 6-Dimethamidodibenzopyriodonium citrate (I-65) 0.5 and 1 mg.kg-1 pretreatment reduced the size of myocardial infarct after ischemia for 40 min and reperfusion for 120 min.

Differential distribution of glycoconjugates in human reproductive tract.

Objectives: To study the distribution of glycoconjugates in the epithelia of various regions of the human female reproductive tract and to examine whether lectins can be used as specific probes to define cell populations in the human female reproductive tract.

Design: Nineteen fluorescein isothiocyanate (FITC)-labeled lectins, which recognize different oligosaccharide structures, were applied to frozen sections of human fallopian tubes (distal and proximal segments) and endometrium. The differential binding patterns of the lectins to the epithelia were evaluated under fluorescent microscopy.

Protection of bone-marrow granulocyte-macrophage colony-forming units in mice bearing in vivo alkylating-agent-resistant EMT-6 tumors.

The survival of bone-marrow granulocyte-macrophage colony-forming units (CFU-GM), an alkylating-agent-sensitive normal tissue, was assessed in mice bearing the EMT-6 parental tumor or the in vivo resistant EMT-6/CDDP, EMT-6/CTX, EMT-6/Thio, and EMT-6/Carbo tumors. The survival pattern of the bone-marrow CFU-GM recapitulated the survival of the tumor cells, mimicking the development of resistance and reversion to sensitivity upon removal of the selection pressure for each of the four alkylating agents. When the EMT-6 parental tumor was implanted in the opposite hind limb of animals bearing the EMT-6/CDDP or EMT-6/CTX tumor, the survival of the parental tumor cells after treatment of the animals with the appropriate antitumor alkylating agent was enhanced.

[Intracellular bile acid activities in colon cancer and normal colon mucosa].

Bile acids are thought to be involved in the etiology and development of colo-rectal cancers. In this study, the intracellular bile acid activities both in colo-rectal cancers and normal colo-rectal mucosa in 12 patients were measured with a new bile acid ion selective microelectrodes. The results demonstrated that bile acid activities of colo-rectal mucosa cells were 5.

[Expression of human lymphotoxin gene in CHO-DHFR- cells].

A recombinant plasmid p91-HuLT was constructed with a human lymphotoxin (HuLT) gene 2.4 kb EcoRI fragment and a mammalian cell expression vector plasmid p91023. The HuLT EcoRI DNA fragment covers entire coding sequence and some 3(1) non-translated region sequence of the gene, p91-HuLT DNA was used to transfect Chinese hamster cell line CHO-DHFR- cells by using calcium phosphate-DNA coprecipitation method, and DHFR+ transfectants were obtained.