Publications by authors named "J. Cihalikova"
Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent an essential tool for positional cloning. However, wheat BAC libraries comprise more than million clones, which makes their screening very laborious.
View Article and Find Full Text PDFCommon wheat (Triticum aestivum L., 2n = 6x = 42) is a polyploid species possessing one of the largest genomes among the cultivated crops (1C is approximately 17 000 Mb). The presence of three homoeologous genomes (A, B and D), and the prevalence of repetitive DNA make sequencing the wheat genome a daunting task.
View Article and Find Full Text PDFArticle Synopsis
- The study addresses the challenges of analyzing the complex genome of common wheat due to its large size and allohexaploid structure, proposing a strategy to dissect it into individual chromosomes.
- The researchers successfully sorted and cloned chromosome 3B using flow cytometry, creating a bacterial artificial chromosome (BAC) library with nearly 68,000 clones and comprehensive coverage of the chromosome.
- The innovative method merges flow cytogenetics with genomics, facilitating enhanced wheat genome analysis and validating its effectiveness through targeted characterization of chromosome 3B.