Application of a semipermeable surface column for the determination of amoxicillin in human blood serum.

A high-performance liquid chromatography column-switching system for the automated determination of amoxicillin in human serum was developed as a more efficient alternative for the already existing systems with off-line sample pretreatment. The column-switching system consists of a semipermeable surface (SPS) column and an analytical reversed-phase (RP) C18 column. After centrifuging, pure serum samples were injected into the column-switching system.

Determination of amoxicillin in human plasma by high-performance liquid chromatography and solid phase extraction.

A high-performance liquid chromatographic method using solid phase extraction has been developed for the determination of amoxicillin in human plasma. After concentration of amoxicillin on a C8 cartridge, the cartridge was eluted on-line to a reversed-phase column packed with 5 microns Chromspher C18. The mobile phase was methanol-0.