Membrane-embedded CdaA is required for efficient synthesis of second messenger cyclic di-AMP.

Cyclic di-adenylate monophosphate (cyclic di-AMP) is an important second messenger in microorganisms. Cyclic di-AMP regulates bacterial cell volume and turgor via control of potassium and compatible solute transport but is also involved in many other processes, including the activation of the metazoan innate immune response to bacterial infections. We compare the activity of full-length membrane-embedded CdaA, the enzyme that synthesizes cyclic di-AMP, with the water-soluble catalytic domain CdaA-DAC.

Becoming skeptical towards vaccines: How health views shape the trajectories following health-related events.

Recent studies on skepticism towards childhood vaccination urge scholars to analyse vaccination trajectories. Focusing on a social group that recent studies point out as being especially relevant because of its relatively high level of skepticism toward childhood vaccination, we use in-depth interviews resembling open conversations to explore how more-educated parents' views on vaccination came about. Providing an in-depth understanding of these vaccine-skepticism trajectories, we additionally analyse 1) how health-related events play a role in parents' trajectories, and 2) how these trajectories are shaped by parents' pre-existing health views.

"Following Your Gut" or "Questioning the Scientific Evidence": Understanding Vaccine Skepticism among More-Educated Dutch Parents.

This study aims to understand vaccine skepticism among a population where it is remarkably prevalent-more-educated Dutch parents-through 31 in-depth interviews. Whereas all respondents ascribe a central role to the individual in obtaining knowledge (i.e.