Significant difference in femoral torsion between coronal plane alignment of the knee type 1 and 4.

Purpose: The purpose of this study was to find out whether the torsions of the femur and tibia are dependent on the coronal plane alignment of the knee (CPAK) type.

Methods: Five hundred patients (1000 legs) were included, who received a whole leg standing three-dimensional (3D) radiograph using EOS imaging (EOS Imaging, Paris, France). SterEOS software was used for digital reconstruction.

Immunoturbidimetric method for routine determinations of apolipoproteins A-I, A-II, and B in normo- and hyperlipemic sera compared with immunonephelometry.

We describe a method for routine immunoturbidimetry of apolipoproteins (apo) A-I, A-II, and B in both normo- and hyperlipemic sera. A special antiserum reagent, consisting of a highly concentrated mixture of nonionic and anionic detergents (final concentration in the assay, 36 g/L), rapidly removes intrinsic turbidities of even strongly lipemic sera without interfering with the antigen-antibody precipitation reaction. The method has good precision, and obviates the need for special sample pretreatment, extended incubation periods, and measurment of sample blanks.

Precipitation methods for the determination of LDL-cholesterol.

The selective precipitation of low-density lipoproteins (LDL) with polyvinyl sulfate (PVS), and the immunoprecipitation of high-density lipoproteins (HDL) and very-low-density lipoproteins (VLDL) with an anti-HDL antibody, can both be used to establish simple methods for the determination of LDL cholesterol. Whereas the PVS method requires the calculation of LDL cholesterol as the difference of total and supernatant cholesterol, the immunoprecipitation method allows the direct measurement of LDL cholesterol in the supernatant. As a first step, both methods were optimized to yield accurate values for normolipemic and slightly hyperlipemic serum samples.

A new approach to photometry of glycated hemoglobin in human blood.

This new approach to measurement of glycated hemoglobin (Hb A1) is a three-step procedure involving (a) conversion of oxyhemoglobin into NO-hemoglobin by sodium dithionite and sodium nitrite in the presence of inositol hexaphosphate; (b) incubation with haptoglobin, which preferentially binds glycated hemoglobin; and (c) determination of the hemoglobin/haptoglobin complex by its peroxidase activity in acid buffer. The procedure is suitable for automated analysis with instruments that are capable of adding a starting reagent. We describe preliminary adaptations for the Abbott VP and Hitachi 705 analyzers and demonstrate the correlation of results with Hb A1 concentrations determined by ion-exchange chromatography.