Publications by authors named "J Zenker"
Circular RNA (circRNA) is covalently closed, single-stranded RNA produced by back-splicing. A few circRNAs have been implicated as functional; however, we lack understanding of pathways that are regulated by circRNAs. Here we generated a pooled short-hairpin RNA library targeting the back-splice junction of 3,354 human circRNAs that are expressed at different levels (ranging from low to high) in humans.
View Article and Find Full Text PDFCellular mechanisms of monozygotic twinning: clues from assisted reproduction.
Hum Reprod Update
December 2024
Background: Monozygotic (MZ) twins are believed to arise from the fission of a single fertilized embryo at different stages. Monochorionic MZ twins, who share one chorion, originate from the splitting of the inner cell mass (ICM) within a single blastocyst. In the classic model for dichorionic MZ twins, the embryo splits before compaction, developing into two blastocysts.
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- Alpha, beta, and gamma tubulins are crucial for all eukaryotic cells, but the roles of other tubulins like delta, epsilon, and zeta are not well understood.
- In a study using mice, researchers focused on epsilon tubulin (TUBE1) and found it is vital for various microtubule structures, particularly in spermatogenesis.
- The absence of TUBE1 resulted in significant germ cell loss and male sterility, highlighting its importance and suggesting a connection with katanin that helps regulate microtubule structures.
Article Synopsis
- The study characterizes atmospheric aerosol analogs in a lab setting, focusing on how varying concentrations of NaCl in these aerosols affect their optical properties.
- Light interactions with these aerosols lead to scattering and absorption, which can be analyzed using Mie theory and depend on droplet size and composition.
- Experiments reveal that altering the NaCl concentration significantly influences both the physical and optical features of the aerosols, allowing for potential adjustments to their properties.
The spatial sorting of RNA transcripts is fundamental for the refinement of gene expression to distinct subcellular regions. Although, in non-mammalian early embryogenesis, differential RNA localisation presages cell fate determination, in mammals it remains unclear. Here, we uncover apical-to-basal RNA asymmetries in outer blastomeres of 16-cell stage mouse preimplantation embryos.
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