Publications by authors named "J Z Cieslinski"
Article Synopsis
- Bone cement with antibiotics like vancomycin or gentamicin is used to fight orthopedic infections, but their effectiveness is limited against some bacteria.
- This study tested silver nanoparticles (AgNPs) combined with polymethylmethacrylate (PMMA) to see if they could reduce biofilm formation by several bacteria, including Staphylococcus aureus and Pseudomonas aeruginosa.
- Results showed that while AgNP-loaded PMMA with antibiotics significantly decreased bacteria counts, AgNPs alone weren't as effective, highlighting the importance of using them together for better results against stubborn infections.
Introduction: Urinary catheter-related infection is commonly associated with bacterial biofilm. The impact of anaerobes is unknown, but their detection in the biofilm on this device has not been previously reported. This study aimed to evaluate the capability to recovery strict, facultative, and aerobic microorganisms in patients using bladder catheters from ICUs using conventional culture, sonication, urinary analysis, and mass spectrometry.
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- - This study investigated how different levels of vancomycin and gentamicin in polymethylmethacrylate (PMMA) can reduce biofilm formation by Staphylococcus aureus.
- - Researchers used various methods, including quantitative analysis and microscopy, to assess the effectiveness of the antibiotic-loaded PMMA, finding significant reductions in colony counts (p < 0.05).
- - The combination of vancomycin (4 g) and gentamicin (500 mg) showed the best results in inhibiting bacterial growth and demonstrated a synergistic effect, indicating potential for use in antibiotic-loaded bone cement.
Article Synopsis
- A retrospective study evaluated the effectiveness of qPCR for detecting infections in orthopedic implants by analyzing 73 sonicated fluid samples from infected patients.
- The study found that while there were no significant differences in patient demographics, those who used antibiotics for longer before device removal had lower culture positivity rates.
- The qPCR results indicated high sensitivity with a cycle threshold cut-off of 26.89 and could detect bacterial DNA in all samples, but antibiotic presence may lead to overestimating infection diagnoses despite not affecting qPCR results.
The impact of VITEK 2 implementation for identification and susceptibility testing of microbial isolates in a Brazilian public hospital.
J Med Microbiol
June 2022
The use of automated systems in identification and susceptibility tests can improve antimicrobial therapy, and positively impact clinical outcomes with a decrease in antimicrobial resistance, hospitalization time, costs, and mortality. The aim of this study was to evaluate the clinical impact of an automated method for identification and susceptibility testing of microbial isolates. This was a retrospective cross-sectional study aimed to analyse the results before and after the implementation period of a VITEK 2 system in a Brazilian university hospital.
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