Publications by authors named "J Wojnacki"
Article Synopsis
- RhoA has a dual role in axon development, acting both as an inhibitor and a promoter, depending on the timing and specific downstream signaling pathways involved.
- In undifferentiated neurons, RhoA activity is concentrated in growth cones, while in developing axons, it shows a biphasic pattern: low in nascent axons and heightened in elongating ones.
- Different RhoA signaling pathways influence axon growth: RhoA-ROCK signaling prevents axon initiation but doesn’t affect elongation, whereas RhoA-mDia signaling enhances elongation by stabilizing microtubules in growth cones.
Agonist-mediated stimulated pathway of mucin and insulin release are biphasic in which rapid fusion of pre-docked granules is followed by slow docking and fusion of granules from the reserve pool. Here, based on a cell-culture system, we show that plasma membrane-located tetraspanin-8 sequesters syntaxin-2 to control mucin release. Tetraspanin-8 affects fusion of granules during the second phase of stimulated mucin release.
View Article and Find Full Text PDFWe show that TANGO2 in mammalian cells localizes predominantly to mitochondria and partially at mitochondria sites juxtaposed to lipid droplets (LDs) and the endoplasmic reticulum. HepG2 cells and fibroblasts of patients lacking TANGO2 exhibit enlarged LDs. Quantitative lipidomics revealed a marked increase in lysophosphatidic acid (LPA) and a concomitant decrease in its biosynthetic precursor phosphatidic acid (PA).
View Article and Find Full Text PDFThe neuropeptide VGF was recently proposed as a neurodegeneration biomarker. The Parkinson's disease-related protein leucine-rich repeat kinase 2 (LRRK2) regulates endolysosomal dynamics, a process that involves SNARE-mediated membrane fusion and could regulate secretion. Here we investigate potential biochemical and functional links between LRRK2 and v-SNAREs.
View Article and Find Full Text PDFThe COVID-19 pandemic has posed and is continuously posing enormous societal and health challenges worldwide. The research community has mobilized to develop novel projects to find a cure or a vaccine, as well as to contribute to mass testing, which has been a critical measure to contain the infection in several countries. Through this article, we share our experiences and learnings as a group of volunteers at the Centre for Genomic Regulation (CRG) in Barcelona, Spain.
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