Thrombosis, Translational Medicine, and Biomarker Research: Moving the Needle.

Arterial and venous thromboembolism are leading causes of morbidity and death worldwide. Despite significant advances in the diagnosis, prognostication, and treatment of thrombotic diseases over the past 3 decades, the adoption of findings stemming from translational biomarker research in clinical practice remains limited. Biomarkers provide an opportunity to enhance our understanding of pathophysiological processes and optimize treatment strategies.

Traffic safety at level crossings in the Czech Republic.

The article deals with the issue of evaluation of the causes of accidents at level crossings in the Czech Republic, including the prediction of the development and proposals of measures for prevention and thus reduction of their number. The goal of this work is to verify the hypothesis that the number of accidents at level crossings in the Czech Republic is decreasing. The authors use available data from the Ministry of Transport and process statistical data for ten years (2013-2022) of railway operation.

Analyzing Extracellular Vesicle-associated DNA Using Transmission Electron Microscopy at the Single EV-level.

Extracellular vesicles (EVs) play an important role in cell-cell communication, carrying bioactive molecules including DNA. EV-associated DNA (EV-DNA) has created enormous interest in the field of biomarkers, particularly related to liquid biopsy. However, its analysis is challenging due to the nanoscale structure of EVs, the low abundance of EV-DNA, and surrounding biogenetic debate.

Light-Controlled Anticancer Activity and Cellular Uptake of a Photoswitchable Cisplatin Analogue.

A photoactive analogue of cisplatin was synthesized with two arylazopyrazole ligands, able to undergo -/- photoisomerizations. The photoisomer showed a dark half-life of 9 days. The cytotoxicities of both photoisomers of the complex were determined in several cancer and normal cell lines and compared to that of cisplatin.