A method for perfusion of the isolated rabbit liver through the portal vein and the hepatic artery in a recirculating system.

We describe a method for the isolation and recirculating perfusion of rabbit livers. Livers were perfused simultaneously through the portal vein and the hepatic artery. Precise descriptions of the surgical preparation, perfusion apparatus and perfusion method are given.

Influence of dietary calcium:phosphorus ratio on mineral excretion and nephrocalcinosis in female rats.

The effects of dietary calcium (Ca) concentration and calcium: phosphorus (Ca:P) ratio on mineral balance and nephrocalcinosis were studied in female rats. In the first experiment there were two dietary Ca concentrations (0.25 and 0.

Internal hydrocephalus, optic nerve aplasia, and microphthalmia in CPB-WE (Wezob) and Cpb:WU (Wistar) rats.

Wistar rats of the inbred CPB-WE and the random bred Cpb:WU strains have been used in neuroanatomical studies. In both groups of rats a high incidence of hydrocephalus was observed. In the CPB-WE rats the ventricular dilatation was accompanied by optic tract lesions and microphthalmia.

The 'normalization' of germ-free rabbits with host-specific caecal microflora.

Hysterectomy-derived germ-free rabbits were given colonization-resistant caecal flora (CRF) from mice, or microflora obtained from the caecum of an antibiotic-decontaminated conventional rabbit and compared with rabbits conventionally raised with the doe. Bodyweight and the following intestinal parameters were determined for the 3 groups: colonization resistance to E. coli, relative caecal weight, villus:crypt ratio (ileum), beta-aspartylglycine (faeces), volatile fatty acids (caecum), and bile acids (faeces).