Publications by authors named "J W Leong"
Quality by Design in continuous bioprocessing: Investigating the impact of critical process parameters of multi-column chromatography during steady-state and transient phases.
J Chromatogr A
December 2024
Given the complexities of continuous bioprocessing, it is critical to thoroughly investigate the process parameters unique to multi-column chromatography (MCC) and their potential impacts. However, existing studies have focused on either loading densities or residence time at steady states only, and their combined impact on critical quality attributes (CQAs) especially during transient phases were less known. In this study, we investigated the impact of critical process parameters during both steady-state and transient phases (start-up, close-down, and intermediate perturbation) through full factorial design.
View Article and Find Full Text PDFIn this work, we developed PolyMap (polyclonal mapping), a high-throughput method for mapping protein-protein interactions. We demonstrated the mapping of thousands of antigen-antibody interactions between diverse antibody libraries isolated from convalescent and vaccinated COVID-19 donors and a set of clinically relevant SARS-CoV-2 spike variants. We identified over 150 antibodies with a variety of distinctive binding patterns toward the antigen variants and found a broader binding profile, including targeting of the Omicron variant, in the antibody repertoires of more recent donors.
View Article and Find Full Text PDFEarly versus delayed timing of vitrectomy after open-globe injury.
Cochrane Database Syst Rev
November 2024
Article Synopsis
- This protocol outlines a Cochrane Review focused on the timing of vitrectomy following open-globe injuries.
- The main goal is to compare the effects of performing the surgery early versus later on patients' visual outcomes.
- The review aims to provide evidence that could guide clinical decisions regarding the optimal timing for vitrectomy in such cases.
Article Synopsis
- Mentoring is essential for professional identity formation (PIF), as it fosters personalized relationships and integrates program values, though its mechanisms remain unclear.
- A systematic scoping review examined existing literature on mentoring support from 2000 to mid-2023, using rigorous methodology to analyze themes and categories.
- Key findings highlight four domains vital to effective mentoring support: definitions and roles, personalisation, shepherding, and PIF, showcasing the importance of adapting mentoring approaches to meet individual mentee needs.
Article Synopsis
- Parkinson's disease (PD) is a progressive movement disorder becoming more common due to an aging population, and researchers aimed to explore rare genetic variants that could help explain its development.
- Whole-exome sequencing was conducted on a large group of PD cases and controls of Asian ancestry, revealing significant links between the genes GBA1 and SMPD1 and the risk of developing PD, confirmed in additional samples.
- The research found that specific SMPD1 variants that reduced enzyme activity were particularly associated with PD risk, with a prominent Asian-specific variant being common among carriers.