Publications by authors named "J W Alexander Elferink"
Molecular methods offer fast, safe and cost-efficient detection of pathogenic bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). These tests depend on a rapid extraction of bacterial DNA. The aim of this study was to compare an optimized DNA extraction and purification protocol for MRSA using magnetic nanoparticles with the original method.
View Article and Find Full Text PDFScreening methods for the detection of antibiotic residues in slaughter animals: comparison of the European Union Four-Plate Test, the Nouws Antibiotic Test and the Premi®Test (applied to muscle and kidney).
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
January 2011
Microbial growth inhibition tests are widely used as the primary screening approach for the detection of antibiotic residues in slaughter animals. In this study we evaluated and compared the performance of the European Union Four-Plate Test (EU4pt), the Nouws Antibiotic Test (NAT), and a commercial ampoule test, the PremiTest (applied to both muscle and kidney), by parallel analysis of 735 slaughter animals. The EU4pt only showed significant inhibition with two muscle samples containing 305 µg kg(-1) doxycycline and 648 µg kg(-1) tulathromycin, while an maximum residue limit (MRL) violation of 1100 µg kg(-1) sulfamethazine remained unnoticed.
View Article and Find Full Text PDFApplication of a luminescent bacterial biosensor for the detection of tetracyclines in routine analysis of poultry muscle samples.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
August 2010
Tetracyclines are extensively used in veterinary medicine. For the detection of tetracycline residues in animal products, a broad array of methods is available. Luminescent bacterial biosensors represent an attractive inexpensive, simple and fast method for screening large numbers of samples.
View Article and Find Full Text PDFComparison of a fluoroquinolone surface plasmon resonance biosensor screening assay with established methods.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
April 2009
Article Synopsis
- - The study compared a new immunochemical biosensor method for detecting fluoroquinolone antibiotics in poultry, fish, and eggs with traditional testing methods.
- - Blank samples were spiked with antibiotics to check method effectiveness, and homogeneity was confirmed via liquid chromatography-mass spectrometry (LC-MS/MS).
- - The new biosensor successfully identified all contaminated samples and was found to be more sensitive and faster than the microbiological assay used for comparison.
Background: The massive pulmonary neutrophil influx in respiratory distress syndrome (RDS) in preterm infants has been ascribed to the effect of leukotriene B(4) (LTB(4)).
Objectives: To investigate whether secretory phospholipase A(2) (sPLA(2)), the rate-limiting enzyme in LTB(4) production, is present in lungs of RDS infants and stimulates neutrophil migration.
Methods: sPLA(2) was measured in tracheal aspirates from 15 preterm infants with RDS.