Rescuing the Right Ventricle: Mechanical Support After Pediatric Heart Transplantation.

Background: Right ventricular (RV) failure after heart transplantation (HT) is common in those with pretransplantation elevated pulmonary vascular resistance (PVR). Mechanical circulatory support has been used as a bridge to recovery, with mixed outcomes. We describe a patient with failed single-ventricle palliation in whom severe RV failure developed after HT.

A concept analysis of the recurrence-related to diabetic foot ulcers.

Background And Purpose: There is inconsistency and lack of conceptual clarity regarding the concept of "recurrence" in diabetes-related foot ulcers. Therefore, the purpose of this concept analysis is to analyze the concept of recurrence-related to diabetes-related foot ulcer after the healing of a previous foot ulcer.

Method: The Rodger's evolutionary concept analysis method was employed to analyze the concept of recurrence in diabetes-related foot ulcers.

Changes of health-related quality of life within 6 months after stroke by clinical and sociodemographic factors.

Stroke causes functional disabilities and reduces the quality of life of stroke survivors. This retrospective cohort study aimed to examine changes in health-related quality of life (HRQoL) six months after a stroke and the effect of stroke characteristics and sociodemographic factors on HRQoL. Participants completed a questionnaire that included the stroke characteristics, sociodemographic characteristics, and the 5-level European Quality of Life 5 dimensions (EQ-5D-5L) before discharge.

Characterisation of the Cytochrome P450 Monooxygenase CYP116B46 from Tepidiphilus thermophilus as a Homogentisic Acid Generating Enzyme and its Conversion to a Peroxygenase.

The heme enzymes of the cytochrome P450 superfamily (CYPs) catalyse the selective hydroxylation of unactivated C-H bonds in organic molecules. There is great interest in applying these enzymes as biocatalysts with a focus on self-sufficient CYP 'fusion' enzymes, comprising a single polypeptide chain with the electron transfer components joined to the heme domain. Here we elucidate the function of the self-sufficient CYP116B46 fusion enzyme, from the thermophilic bacterium Tepidiphilus thermophilus.

The biarylitides: understanding the structure and biosynthesis of a fascinating class of Cytochrome P450 modified RiPP natural products.

The biarylitides are a recently discovered class of RiPP natural products that are fascinating both from the small size of the core peptides as well as the diversity of peptide crosslinking exhibited by the cytochrome P450 enzymes found in these systems. In this review, we address the discovery and biosynthetic diversity of these systems and discuss the methods and challenges of analysing the structures of these constrained cyclic peptides. We also discuss the structures of the P450 enzymes involved in these pathways and address the potential for alternate catalytic outcomes and activities as seen most recently with the inclusion of biarylitide related enzymes within rufomycin biosynthesis.