Spatiotemporal metabolic mapping of ex-situ preserved hearts subjected to dialysis by integration of bio-SPME sampling with non-targeted metabolipidomic profiling.

Background: Normothermic ex situ heart perfusion (ESHP) has emerged as a valid modality for advanced cardiac allograft preservation and conditioning prior to transplantation though myocardial function declines gradually during ESHP thus limiting its potential for expanding the donor pool. Recently, the utilization of dialysis has been shown to preserve myocardial and coronary vasomotor function. Herein, we sought to determine the changes in myocardial metabolism that could support this improvement.

Exploring the Potential of Genome-Wide Hybridization Capture Enrichment for Forensic DNA Profiling of Degraded Bones.

Unlabelled: In many human rights and criminal contexts, skeletal remains are often the only available samples, and they present a significant challenge for forensic DNA profiling due to DNA degradation. Ancient DNA methods, particularly capture hybridization enrichment, have been proposed for dealing with severely degraded bones, given their capacity to yield results in ancient remains.

Background/objectives: This paper aims to test the efficacy of genome-wide capture enrichment on degraded forensic human remains compared to autosomal STRs analysis.

A Prospective Study of the Prevalence and Predictive Risk Factors of Repeat Breeder Syndrome in Dairy Cattle in the North of Spain.

This study examined the prevalence and risk factors of repeat breeder syndrome (RB) in 2370 dairy cows in northern Spain. Data collected included the prevalence of postpartum pathologies, metabolic markers, and productive and reproductive parameters. The overall RB prevalence was 21.

Quantification of modal mineralogy in molybdenite-bearing drill-core samples by laser-induced breakdown spectroscopy.

Quantification of modal mineralogy in drill-core samples is crucial for understanding the geology and metal deportment in a mining operation. This study assesses conventional procedures to quantify modal mineralogy, that includes an initial drill-core logging, followed by petrographic descriptions and SEM-based automated mineralogy analyses performed in selected regions of interest, against a novel approach using laser-induced breakdown spectroscopy (LIBS). Our proposed methodology aims to quantify the modal mineralogy directly in a drill-core sample, avoiding previous stages of selection and preparation of samples.

Marijuana Poisoning in Canines in the Aburrá Valley (Antioquia-Colombia), 2023-2024.

Marijuana poisoning in canines is still considered a taboo topic. Poisoning in pets is becoming increasingly common, in many cases resulting in severe clinical signs, such as ataxia, urinary incontinence, mydriasis, depression, and hyperesthesia. Colombia does not have any reliable test for the diagnosis of exposure to cannabis in animals, and it is not an obligation to report this kind of poisoning to the authorities.