Quantitative Chemical Imaging of Organelles.

ConspectusDNA nanodevices are nanoscale assemblies, formed from a collection of synthetic DNA strands, that may perform artificial functions. The pioneering developments of a DNA cube by Nadrian Seeman in 1991 and a DNA nanomachine by Turberfield and Yurke in 2000 spawned an entire generation of DNA nanodevices ranging from minimalist to rococo architectures. Since our first demonstration in 2009 that a DNA nanodevice can function autonomously inside a living cell, it became clear that this molecular scaffold was well-placed to probe living systems.

Cellular Activity of Typhimurium ArtAB Toxin and Its Receptor-Binding Subunit.

Salmonellosis is among the most reported foodborne illnesses in the United States. The Typhimurium DT104 phage type, which is associated with multidrug-resistant disease in humans and animals, possesses an ADP-ribosylating toxin called ArtAB. Full-length AB has been found on a number of broad-host-range non-typhoidal species and serovars.

The Irish Atlantic CoCliME case study configuration, validation and application of a downscaled ROMS ocean climate model off SW Ireland.

This ocean numerical modelling study is focused on the shelf waters off southwest Ireland. Outputs from the model are used to provide scientists and policy makers with climate change information related to oceanic conditions that influence harmful algal blooms in the region. Four simulations were developed to include a 20-year hindcast simulation (1997-2016), a 31-year historical simulation (1975-2005), and two 30-year future climate model projections (2006-2035) for the representative carbon pathways (RCP) 4.

Evaluation of the Efficacy of a Cholera-Toxin-Based Vaccine against Bovine Intramammary Challenge.

() is a primary agent of bovine mastitis and a source of significant economic loss for the dairy industry. We previously reported antigen-specific immune induction in the milk and serum of dairy cows following vaccination with a cholera toxin A and B subunit (CTA/B) based vaccine containing the iron-regulated surface determinant A (IsdA) and clumping factor A (ClfA) antigens of (IsdA + ClfA-CTA/B). The goal of the current study was to assess the efficacy of this vaccine to protect against infection after intramammary challenge.