Exploring novel antifungal peptides from peptic hydrolysis of chicken cruor protein via regression-based machine learning approach.

There is a growing interest in natural preservatives driven by consumer demand for clean-label products. In Canada, approximately 48 million liters of blood are produced annually during chicken slaughter, offering an opportunity to valorize cruor, the solid blood component rich in hemoglobin, for use in food preservation. This study investigated the hydrolysis of chicken cruor with pepsin at pH 2, 3, 4, and 5 for 180 min to produce antimicrobial peptides.

Guideline-Directed Medical Therapy After Hospitalization for Acute Heart Failure: Insights From the CONNECT-HF.

Background: Significant gap remains in the implementation of guideline-directed medical therapy (GDMT) in patients with heart failure after a hospitalization. We aimed to evaluate the use and titration of GDMT at discharge and over a 12-month period after hospital discharge and to identify factors associated with GDMT use and titration.

Methods And Results: The CONNECT-HF (Care Optimization Through Patient and Hospital Engagement Clinical Trial for Heart Failure) trial evaluated the effect of a hospital and postdischarge quality improvement intervention in participants with heart failure with reduced ejection fraction.

Upcycling calf cruor slaughterhouse by-product by peptic hydrolysis and identification of three novel antifungal peptides following a regression based-machine learning approach.

Calf cruor (C-cru) is an understudied source of antimicrobial peptides that can be used for meat product biopreservation under a circular economy framework. This study assessed the impact of pH and peptic hydrolysis duration of C-cru on the enzymatic mechanism, peptide population, and antimicrobial activities (antibacterial, antifungal, and anti-yeast). The results showed that peptic hydrolysis of C-cru has similar enzymatic mechanisms to adult bovine hemoglobin, fostering the development of a zipper mechanism at pH 2 and 3, while the one-by-one mechanism is promoted at pH 4 and 5.