HET-CAM test for determining the possible eye irritancy of pesticides.

Agrochemicals and veterinary products must undergo numerous toxicological tests before registration. The use of animals in these studies is a controversial issue. The Draize eye irritation test is one of the most criticised methods because of the injuries inflicted on the test animals.

An alternative test battery in detecting ocular irritancy of agrochemicals.

Using animals in toxicological screening is a controversial issue. To get knowledge about eye irritation, recently only the in vivo Draize-test is accepted, which is one of the most criticized methods because of the injuries inflicted on the test animals. In recent years various in vitro methods have been developed to replace the primary irritation test.

Eye irritation study of some pesticides on chorioallantoic membrane of the egg.

The chorioallantoic membrane of chick embryo has been used extensively for many years in various fields of biological research, including virology, bacteriology and toxicology. The chorioallantoic membrane (CAM) is a complete tissue that responds to injury with a complete inflammatory reaction, this process similar to that induced by chemicals in the conjunctival tissue of the rabbit eye. A possible model for assessing the irritation potential of a chemical or product to such a vascularised tissue is the choriallantoic membrane of the embryonated hen's egg, as this is a highly vascular, thin membrane with relatively easy access for both treatment and assessment.

The use of HET-CAM test in detecting the ocular irritation.

Agrochemicals must undergo numerous toxicological tests before registration. One of these experiments is the examination of eye irritation potential. To get knowledge about eye irritation, recently only the in vivo Draize-test is accepted, which is one of the most criticized methods because of the injuries inflicted on the test animals.

Toxicity study of agrochemicals on chorioallantoic membrane of the egg.

Using animals in toxicological screening is a controversial issue. The Draize eye irritation test is one of the most criticized methods because of the injuries inflicted on the test animals. That's why several various tests have been developed to replace the rabbits in detecting the irritation potential of chemicals.