Type I collagen carboxyterminal telopeptide in human gingival crevicular fluid in different clinical conditions and after periodontal treatment.

A total of 126 gingival crevicular fluid (GCF) samples were collected from 20 adults using paper strips. Patients were divided into a periodontitis-affected group (13 subjects) and a periodontitis-free group (7 subjects) by pocket depth and radiological bone loss. 4 subjects from the periodontitis-affected group received a single episode of periodontal treatment (scaling, root planing and curettage) and GCF samples were collected 2, 5, 10, 20 and 40 days after treatment.

Molecular forms and concentration of fibronectin and fibrin in human gingival crevicular fluid before and after periodontal treatment.

A total of 23 periodontitis-affected sites from seven adults was selected for the study. Crevicular fluid (CF) samples were collected with paper strips before treatment (scaling, root planing, and curettage) and 2, 5, 10, 20, and 40 days after treatment. Each sample was eluted into sterile saline and two aliquots were drawn for gel electrophoresis: one for fibronectin and one for fibrin analysis.

Collagen I carboxyterminal propeptide in human gingival crevicular fluid before and after periodontal treatment.

The amount of procollagen I carboxyterminal propeptide (PICP) in crevicular fluid (CF) was measured in three periodontitis patients. Samples were collected from 29 sites before treatment (scaling, root planing, and curettage) and 2, 5, 10, 20, and 40 days after treatment, by placing two paper strips in periodontal pockets for 5 s. The amount of fluid in strips was measured by the Periotron device.

Characterization of fibronectin and fibrin(ogen) fragments in gingival crevicular fluid.

A total of 49 crevicular fluid (CF) samples were collected with paper strips from 12 healthy adults. Each sample was eluted into sterile saline and two aliquots were drawn for SDS-PAGE, one for fibronectin and one for fibrin analysis. Peptides were transferred to nitrocellulose membranes, and fibronectin and fibrin were detected using specific antibodies.

Changes in amount of gingival crevicular fluid after a single episode of periodontal treatment.

A total of 51 periodontal sites from 6 adults with no systemic diseases or medication were selected for the study. All sites showed radiologic bone loss and pockets of 4 mm or more. Crevicular fluid (CF) was collected by inserting filter paper strips into periodontal pockets for 5 s and was measured by Periotron.