[Development of PhaP-tagged protein expression and purification systems for extremely halophilic archaea ].

[ OBJECTIVE] To establish a convenient halophilic protein expression and purification system based on the haloarchaeal-type PhaP and polyhydroxyalkanoate (PHA) granule. [METHODS] We cloned a strong haloarchaeal promoter and the phaP-tag into the haloarchaea- Escherichia coli shuttle vector pWL502, and then used the constructed vector to express the PhaP-tagged haloarchaeal proteins in the phaP-deleted strain Haloferax mediterranei AphaP. We purified the PhaP-fusion proteins, which were associated with PHA granules, by sucrose density gradient centrifugation.

Factors determining calcium intake in elderly women of Appalachia.

Background: Although it has been recommended that women aged 65 and older consume at least 1,500 mg of calcium daily, no studies are available that define the factors associated with adequate intake.

Methods: We studied 184 elderly women to determine the relationship between foodstuff calcium consumption and anthropomorphic measurements and numerous life-style changes.

Results: The mean foodstuff calcium consumption was 563.