Nucleo-cytoplasmic environment modulates spatiotemporal p53 phase separation.

Liquid-liquid phase separation of various transcription factors into biomolecular condensates plays an essential role in gene regulation. Here, using cellular models and in vitro studies, we show the spatiotemporal formation and material properties of p53 condensates that might dictate its function. In particular, p53 forms liquid-like condensates in the nucleus of cells, which can bind to DNA and perform transcriptional activity.

Single-Cell and Spatial Multi-omics Reveal Interferon Signaling in the Pathogenesis of Perianal Fistulizing Crohn's Disease.

Background & Aims: Perianal fistulizing Crohn's disease (PCD) is a common and debilitating complication with elusive pathophysiology. We examined mucosal cells from patients with PCD and related conditions using a multi-omics approach.

Methods: We recruited patients with PCD (n = 24), CD without perianal disease (NPCD, n = 10), and idiopathic perianal fistulas (IPF, n = 29).

The Impact of Timing of Voice Therapy in the Vocal Outcomes of Surgically Treated Benign Vocal Fold Lesion Patients.

This study aimed to examine the impact of the timing of voice therapy on vocal outcomes in patients post-surgery for benign vocal fold lesions.The study prospectively assessed 20 patients undergoing phonomicrosurgery for benign vocal fold lesions. Participants were categorized into early voice therapy (within 7 days), late voice therapy (after 7 days), and no voice therapy groups.

Evaluating efficacy and safety of laser interstitial thermal therapy in patients with newly diagnosed and recurrent glioblastoma: a systematic review and meta-analysis.

Glioblastoma (GB), the most common malignant brain tumour, has a poor prognosis despite advances in treatment. Standard management involves surgery followed by chemoradiotherapy. MRI-guided laser interstitial thermal therapy (LITT) is a minimally invasive technique that may offer an option for select patients with specific clinical profiles.