Purification and biochemical characterization of the G4 resolvase and DNA helicase FANCJ.

G-quadruplex (G4) DNA or RNA poses a unique nucleic acid structure in genomic transactions. Because of the unique topology presented by G4, cells have exquisite mechanisms and pathways to metabolize G4 that arise in guanine-rich regions of the genome such as telomeres, promoter regions, ribosomal DNA, and other chromosomal elements. G4 resolvases are often represented by a class of molecular motors known as helicases that disrupt the Hoogsteen hydrogen bonds in G4 by harnessing the chemical energy of nucleoside triphosphate hydrolysis.

Cerium Nanophases from Cerium Ammonium Nitrate.

Ceria nanomaterials with facile Ce redox behavior are used in sensing, catalytic, and therapeutic applications, where inclusion of Ce has been correlated with reactivity. Understanding assembly pathways of CeO nanoparticles (NC-CeO) in water has been challenged by "blind" synthesis, including rapid assembly/precipitation promoted by heat or strong base. Here, we identify a layered phase denoted with a proposed formula Ce(OH)(NO)·HO ( ≈ 2.

Inspiratory muscle training for mechanically ventilated patients in the intensive care unit: Obstacles and facilitators for implementation. A mixed method quality improvement study.

Background: Mechanically ventilated patients are at risk of developing inspiratory muscle weakness (IMW), which is associated with failure to wean and poor outcomes. Inspiratory muscle training (IMT) is a recommended intervention during and after extubation but has not been widely adopted in Dutch intensive care units (ICUs).

Objectives: The objective of this study was to explore the potential, barriers, and facilitators for implementing IMT as treatment modality for mechanically ventilated patients.

Synthesis, Structure, and Heat Capacity of Some Basic Hydroxohalide Glasses of Zirconium and Hafnium.

This work highlights the synthesis and properties of novel basic hydroxohalide glasses of zirconium and hafnium. The hydroxohalide glasses are M(OH)X·(n)HO where M represents either zirconium or hafnium, and X represents either chloride or bromide. The chemical structure is investigated using X-ray diffraction, total scattering, and the pair distribution function method to identify the local structure and any short-range connectivity.

The RNA-binding motif protein 14 regulates telomere integrity at the interface of TERRA and telomeric R-loops.

Telomeric repeat-containing RNA (TERRA) and its formation of RNA:DNA hybrids (or TERRA R-loops), influence telomere maintenance, particularly in human cancer cells that use homologous recombination-mediated alternative lengthening of telomeres. Here, we report that the RNA-binding motif protein 14 (RBM14) is associated with telomeres in human cancer cells. RBM14 negatively regulates TERRA expression.