Publications by authors named "J Soki"
Objectives: This study was conducted to measure the prevalence of antibiotic resistance, and corresponding resistance genes among Bacteroides and related genera in a tertiary hospital.
Methods: We examined 138 clinical strains of Bacteroides, Phocaeicola and Parabacteroides species isolated between July 2018 and June 2022. Antibiotic susceptibility tests were conducted using agar dilution.
Objectives: Bacteroides fragilis is the most frequent cause of anaerobic bacteraemia. Although recent data suggest a rise in antimicrobial resistance (AMR) of this and other anaerobic bacteria, surveillance remains limited due to a lack of both data availability and comparability. However, a newly introduced standardised method for antimicrobial susceptibility testing (AST) of anaerobic bacteria has made larger scale surveillance possible for the first time.
View Article and Find Full Text PDFThe aim of this prospective pilot study was to compare culture and microbiome results of the removed tonsils of patients with assumed distant focal disease (11 patients) and those who underwent a tonsillectomy, due to other reasons, such as recurrent tonsillitis, tonsil stones or snoring (nine patients). Aerobic culture was carried out for samples taken from the surface of the tonsils by swabs before tonsillectomy for all 20 patients. The squeezed detritus and the tissue samples of removed tonsils, taken separately for the right and left tonsils, were incubated aerobically and anaerobically.
View Article and Find Full Text PDFArticle Synopsis
- The study focuses on the Bacteroides fragilis group (BFG), which are significant anaerobic pathogens known for their high antibiotic resistance, and aims to compare the antibiotic resistance genes in healthy individuals' microbiota with those from infected patients.
- Researchers found 13 antibiotic resistance determinants in 184 intestinal BFG strains collected from five European countries, revealing differences in gene prevalence compared to previous studies, particularly in mobile antibiotic resistance genes.
- The findings suggest novel characteristics in intestinal Bacteroides strains, indicating differing prevalence of resistance genes based on their microbiota composition and offering insights into their resistance mechanisms.
Previously, we reported that metronidazole MICs are not dependent on the expression levels of genes in strains and we compared the proteomes of metronidazole-resistant laboratory strains to those of their susceptible parent strains. Here, we used RT-qPCR to correlate the expression levels of 18 candidate genes in a panel of selected, clinical gene-positive and -negative strains to their metronidazole MICs. Metronidazole MICs were correlated with the expression of certain tested genes.
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