CYP2C9 polymorphism studies in the Saudi population.

Objective: To determine the prevalence of CYP2C9 polymorphism in normal Saudis (controls), in Saudi patients with venous thrombosis, in patients requiring low dose warfarin (study group) for anticoagulation, and to compare our results to those from other populations.

Methods: Blood from the control and study groups was collected from November 2001 to November 2008. The DNA was extracted, stored at -70°C and later tested for the CYP2C9 polymorphism using established methods.

Inferior vena caval filters: 5 years of experience in a tertiary care center.

Background And Objectives: Interruption of the Inferior Vena Cava (IVC) is recommended in certain cases to prevent Pulmonary Embolism (PE). Reported data on the efficacy and rate of complications vary considerably.

Patients And Methods: We conducted a retrospective analysis of patients who had a temporary or permanent IVC filter inserted at our institution during the past 5 years.

The Saudi Thrombosis and Familial Thrombophilia Registry. Design, rational, and preliminary results.

Objective: To describe the Registry and report preliminary data for the prevalence of 5 prothrombotic gene mutations in the normal Saudi population.

Methods: Blood from consenting healthy Saudi individuals and patients with venous thrombosis (VT) from different regions of the Kingdom was collected from November 2001 until July 2007. The extracted DNA of each sample was kept at -70 degrees C until tested for 5 known prothrombotic factors using established methods.

Comparative study between the Light Cycler and the PCR-restriction fragment length polymorphism in detecting factor V Leiden and factor II 20210G>A mutations.

Objectives: To test reproducibility, speed and cost of testing for factor V Leiden and FII 20210G>A in our practice.

Design And Methods: We compared conformity, reproducibility, speed and cost using the Light Cycler (LC) and PCR-RFLP.

Results: There was 100% conformity and reproducibility.