Ozone does not react with human erythrocyte membrane lipids.

Ozone was applied to sealed red cell ghost membranes at the rate of 95 nmol/min for periods up to 20 min. Acetylcholine esterase, on the outer face of the membrane, was inhibited up to 20%. Glyceraldehyde-3-phosphate dehydrogenase, on the inner surface of the membrane, was inhibited up to 87%.

Reaction of ozone with enzymes of erythrocyte membranes.

Ozone is a widespread component of polluted air. It is the cause of many adverse effects on the lung such as decreased athletic performance and exacerbation of asthma. Ozone inactivated acetylcholine esterase (AChE) both in intact washed human erythrocytes and in ghosts prepared from the erythrocytes.

Activation of eicosanoid metabolism in human airway epithelial cells by ozonolysis products of membrane fatty acids.

Inhaled ozone can react with a variety of cellular macromolecules within the lung. Recent analyses of the chemistry of ozone reactions with unsaturated fatty acids, which are present in all membranes and in mucus in the airways, indicate that ozonolysis yields one aldehyde and one hydroxyhydroperoxide molecule for each molecule of ozone. The hydroxyhydroperoxide molecule is unstable in aqueous environments, and subsequently yields a second aldehyde and hydrogen peroxide.

Physical and chemical characterization of airbag effluents.

Objective: This paper describes a study aimed at characterizing the exposure to physical and chemical by-products from the deployment of airbag restraint systems.

Design, Materials And Methods: Specifically, the levels of particulates and the composition of gases and bag fabric speed were measured in the passenger compartment following deployment of either a driver's side or driver's side/passenger's side airbag system.

Measurements: A Fourier transform infrared analyzer (FTIR) and chemiluminescence analyzers were used for gas analysis, a cascade impactor and gravimetric filter measurements for aerosol determination and high-speed films to determine fabric speed.

Airway epithelial cell responses to ozone injury.

The airway epithelial cells is an important target in ozone injury. Once activated, the airway epithelium responds in three phases. The initial, or immediate phase, involves activation of constitutive cells, often through direct covalent interactions including the formation of secondary ozonolysis products--hydroxyhydroperoxides, aldehydes, and hydrogen peroxide.