Adenine nucleotides and 2,3-dophosphoglycerate in the erythrocytes during physical exercise and restitution in healthy subjects.

The reported investigations were carried out in 6 young (20 years old) healthy males who performed a 10-minute exercise on a Monark cycle ergometer at workloads increasing the heart rate to 170/minute. Before the exercise, after its termination, and after 30 minutes of restitution the concentrations of adenosine-5' triphosphate, adenosine-5' diphosphate, adenosine-5' monophosphate, 2,3-diphosphoglycerate, nicotinamide-adenine dinucleotide and nicotinamide-adenine dinucleotide phosphate were assayed in erythrocytes. After the end of exercise a non-significant increase in ATP concentration and a non-significant decrease of 2,3-DPG were found in the erythrocytes.

Effect of UV-irradiation of shifts of energy-rich phosphate compounds: ADP, ATP and AXP in human red blood cells represented by a trigonometrical polynomial.

This paper deals with the effect of UV-irradiation on metabolism of energy rich adenine, esters: ADP, ATP and AXP in human red blood cells in vitro. Preparation, separation and quantitative determination of these compounds were performed by the method of BARTLETT, using a step concentration gradient with an HC1-NH4C1 chloride system. Blood was irradiated in vitro for 30, 60, 90, 120, 150, 180, 210, 240, 270 and 300 min.

Effect of UV-irradiation in vitro on adenin nucleotides metabolism, Na+ and K+ concentration, osmotic properties and submicroscopic structure of pigeon red blood cells.

Effect of UV-irradiation in vitro on metabolism of adenine nucleotides: ADP, ATP and AXP, osmotic properties and submicroscopic structure of nucleated pigeon red blood cell was investigated. Irradiation carried out for 60, 120, 180, 240 and 300 minutes. Hanau S-500 lamp with efficiency 4.