Publications by authors named "J S Wayland"
Peripheral injury responses essential for muscle repair and nociception require complex interactions of target tissues, immune cells and primary sensory neurons. Nociceptors and myofibers both react robustly to signals generated from circulating immune cells, which promote repair, growth, and regeneration of muscle while simultaneously modulating peripheral sensitization. Here, we found that macrophages form a synaptic-like contact with myofibers to hasten repair after acute incision injury and to facilitate regeneration after major muscle damage.
View Article and Find Full Text PDFObesity is a risk factor for increased lung damage and disease severity during influenza virus infection. White adipose tissue (WAT) inflammation is a key driver of disease pathogenesis in obesity. Whether and how obesity modifies lung and WAT immune cell character and function in obesity to amplify influenza disease severity remains unknown.
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- Vertical transmission of obesity plays a significant role in the ongoing obesity epidemic and related metabolic diseases, yet current models fail to accurately reflect human obesity.
- This study introduces a new mouse model that simulates "human-like" obesity by using a specific diet and housing conditions, revealing that maternal obesity negatively impacts neonatal survival, increases offspring fat accumulation, and heightens their risk for obesity-related diseases.
- The findings suggest that severe maternal obesity alters the offspring's microbiome and creates a harmful inflammatory environment during pregnancy, which is supported by similar patterns observed in a human birth cohort study.
Inflammation-driven preterm birth (PTB) is modeled in mice using lipopolysaccharide (LPS) challenge. Here, we present a protocol for cytokine and uterine immune cell characterization in a mouse model of LPS-induced PTB. We describe steps for LPS challenge, in vivo cytokine capture assay, and isolation of uterine immune cells for flow cytometry.
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- Influenza virus-induced pneumonia poses significant public health risks, with obesity, metabolic diseases, and female sex identified as independent factors worsening the disease.
- The lack of experimental models for studying severe obesity in female mice has limited research, but this study successfully induced severe obesity in female mice using a high-fat diet and thermoneutral housing.
- The findings reveal that while traditionally lean mice show similar responses to influenza, introducing severe obesity and metabolic disease in female mice results in disease severity comparable to that of obese males, highlighting the critical role of these factors in influenza severity.