Publications by authors named "J S Maras"
Background And Aims: Acute liver failure (ALF) has high mortality predominantly due to compromised immune system and increase vulnerability to bacterial and fungal infections.
Method: Plasma lipidome and fungal peptide-based-community (mycobiome) analysis were performed in Discovery cohort (40-ALF, 5-healthy) and validated in a validation cohort of 230-ALF using High-resolution-mass-spectrometry, artificial-neural-network (ANN) and machine-learning (ML).
Results: Untargeted lipidomics identified 2,013 lipids across 8 lipid-groups.
Article Synopsis
- Carcinoma of the gall bladder (CAGB) has a low survival rate, but analyzing bile samples can help identify molecular indicators for early detection of the disease.
- A study on 87 bile samples found specific proteomic and metabolomic signatures associated with CAGB that correlated with increased inflammation and altered energy pathways compared to healthy controls.
- The top four metabolites showed over 99% accuracy and sensitivity for CAGB detection in further validation tests, suggesting that bile analysis could lead to improved early diagnosis methods.
Palmitic acid is the most abundant saturated fatty acid in circulation and causes hepatocyte toxicity and inflammation. As saturated fatty acid can also disrupt the circadian rhythm, the present work evaluated the connection between clock genes and NAD+ dependent Sirtuins in protecting hepatocytes from lipid-induced damage. Hepatocytes (immortal cells PH5CH8, hepatoma cells HepG2) treated with higher doses of palmitic acid (400-600μM) showed typical features of steatosis accompanied with growth inhibition and increased level of inflammatory markers (IL-6 IL-8, IL-1α and IL-1β) together with decline in NAD+ levels.
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- Scientists created a new mouse model to study alcohol-associated hepatitis (AAH) that is more similar to humans than older models.
- They fed male mice a special diet containing alcohol and a chemical called thioacetamide for 12 weeks to induce liver damage.
- The results showed that these mice had major signs of liver problems, inflammation, and changes in gut bacteria, making it a better tool to study AAH compared to other methods.
Background: Patients with pediatric cirrhosis-sepsis (PC-S) attain early mortality. Plasma bacterial composition, the cognate metabolites, and their contribution to the deterioration of patients with PC-S to early mortality are unknown. We aimed to delineate the plasma metaproteome-metabolome landscape and identify molecular indicators capable of segregating patients with PC-S predisposed to early mortality in plasma, and we further validated the selected metabolite panel in paired 1-drop blood samples using untargeted metaproteomics-metabolomics by UHPLC-HRMS followed by validation using machine-learning algorithms.
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