Dietary Chia (Salvia hispanica L.) seeds oil supplementation augments growth performance and gut microbial composition in Labeo rohita fingerlings.

The present study investigates the supplemental effects of chia seed oil (CSO) on the growth performance and modulation of intestinal microbiota in Labeo rohita fingerlings. Four diets were formulated with graded levels of CSO: 1.0%, 2.

Protocol to identify endogenous proximal proteins using biotinylation by antibody recognition.

Biotinylation by antibody recognition (BAR) is an antibody-based approach for mapping proximal protein interactions in cells. Here, we present a protocol to biotinylate and identify proximal proteins using BAR. We describe steps for defining proximity labeling reaction conditions, assessing enrichment using western blot, and sample preparation for mass spectroscopy analysis.

NXP800 activates the unfolded protein response, altering AR and E2F function to impact castration-resistant prostate cancer growth.

Purpose: Advanced prostate cancer (PCa) is invariably fatal with the androgen receptor (AR) being a major therapeutic target. AR signaling inhibitors have improved overall survival for men with advanced PCa, but treatment resistance is inevitable and includes reactivation of AR signaling. Novel therapeutic approaches targeting these mechanisms to block tumor growth is an urgent unmet clinical need.

Unveiling predictors of developmental delay among under-fives: Insights from a community study.

Introduction: The development of a child is a crucial factor that ultimately affects the quality of life of children. Developmental delay can be prevented if it is identified early and adequate attention is given from the start.

Methodology: A cross-sectional analytical study was conducted between December and November 2022 on 450 children by multistage cluster sampling in the Ormanjhi block of Ranchi, Jharkhand.

Early-life gut inflammation drives sex-dependent shifts in the microbiome-endocrine-brain axis.

Despite recent advances in understanding the connection between the gut microbiota and the adult brain, significant knowledge gaps remain regarding how gut inflammation affects brain development. We hypothesized that gut inflammation during early life would negatively affect neurodevelopment by disrupting microbiota communication to the brain. We therefore developed a novel pediatric chemical model of inflammatory bowel disease (IBD), an incurable condition affecting millions of people worldwide.