Antisense-mediated regulation of exon usage in the elastic spring region of Titin modulates sarcomere function.

Background: Alternative splicing of Titin (TTN) I-band exons produce protein isoforms with variable size and elasticity, but the mechanisms whereby TTN splice factors regulate exon usage and thereby determining cardiomyocyte passive stiffness and diastolic function, is not well understood. Non-coding RNA transcripts from the antisense strand of protein-coding genes have been shown to regulate alternative splicing of the sense gene. The TTN gene locus harbours >80 natural antisense transcripts (NATs) with unknown function in the human heart.

Evaluation of Calibration Burden for Monitoring of a Pharmaceutical Continuous Manufacturing Line using Near-Infrared Spectroscopy.

Process analytical technology (PAT) tools are an important part of process monitoring and control in pharmaceutical continuous manufacturing (CM) that help ensure product quality. However, there is hesitancy to adopt PAT due, in part, to the high start-up costs. A portion of the cost is the calibration burden associated with developing an appropriate multivariate data analysis (MVDA) method to extract the desired information from the spectral outputs of spectroscopic PAT tools.

Heritable Genetic Variability in Ovarian Tumours: Exploring Venous Thromboembolism Susceptibility and Cancer Prognosis in a Hospital-Based Study.

Venous thromboembolism (VTE) is a frequently encountered paraneoplastic syndrome in patients with ovarian cancer (OC), an inflamm-aging entity. VTE is known to exacerbate their already poor prognosis, which is partially attributed to the contribution of the haemostatic system to ovarian tumourigenesis. In the past decade, numerous single-nucleotide polymorphisms (SNPs) implicated in VTE pathways have been proposed to influence tumour susceptibility and progression.

Unveiling sex-disparities and the impact of gender-affirming hormone therapy on periodontal health.

Introduction: As personalized medicine advances, the need to explore periodontal health across different sexes and gender identities becomes crucial. This narrative review addresses the gap in understanding how biological sex and gender-affirming hormone therapy (GAHT) influence periodontitis risk.

Results: Research has uncovered significant sex-based immunological disparities driven by X and Y chromosome gene expression and sex-hormones, which may influence susceptibility to periodontitis.